there are many studies related to the production of a elisa kit for diagnosing virus infections. however, production of most kits depends on purification of whole virus particles, which involves the use of costly equipment and reagents. the purpose of this study was to check out if the anti-cp42 antibodies could be used as a diagnostic assay for detection of grapevine fanleaf virus (gflv). in t...

diagnosis of toxoplasma gondii (t.gondii) infection is of great medical importance especially for pregnant women and immunosuppressed patients. numerous studies have shown that the recombinant production of several ttoxoplasma antigens, including dense granule antigens (gras) has a great potential as diagnostic reagents. previous studies reported expression of amino terminal gra8 protein in fus...

We have devloped an enzyme-linked immunosorbent assay for determining choriomammotropin (human placental lactogen) in serum. Unlabeled hormone competes with choriomammotropin-beta-galactosidase conjugate for antibody bound to polystyrene tubes. The entire assay can be performed in 2.5 h with good precision. The coefficient of variation for one sample with a mean concentration of 5.6 mg/L, assay...

results we found 28 cases with positive results for b. melitensis by multiplex pcr technique which was significantly different from of sat (p<0.05). six samples were positive for b. abortus by pcr. conclusion the results of present study showed that multiplex pcr assay is a rapid and sensitive technique for diagnosis of brucellosis compared to sat. however it is more accurate when coupled with ...

conclusions the present findings reveal a relatively high presence of anti-hev iggs and clearly indicate that hev infection is still present in northern algeria. further, the prediction of hev genotype using different antigens generated from the different hev genotypes shows that the causative strains are more likely to be of genotype 1. background two hepatitis e virus (hev) outbreaks occurred...

background rheumatoid arthritis (ra) is the most common chronic inflammatory disease of unknown etiology. in this regard, the role of bacterial superantigens (as an effective agent) were considered. objectives this study aimed to assess staphylococcal enterotoxin e in the blood of patients with rheumatoid arthritis. patients and methods a total of 83 blood samples of patients with ra were studi...