The histaminergic system is thought to regulate brain reward, but the exact mechanisms are poorly understood. This study aims to reveal the pathophysiological differences in the brain histaminergic system between selectively outbred alcohol-preferring AA and alcohol-avoiding ANA rats by using a combination of biochemical, immunohistochemical, and molecular biological methods. We also want to te...

We investigated the effects of the central histaminergic system on afferent sensory signals in the retinogeniculocortical pathway in the intact brain. Extracellular physiological recordings in vivo were obtained from neurons in the cat dorsal lateral geniculate nucleus (LGN) in conjunction with electrical activation of the histamine-containing cells in the tuberomammillary nucleus of the hypoth...

We investigated whether histaminergic tone contributes to the seasonal catabolic state in Siberian hamsters by determining the effect of ablation of histaminergic neurons on food intake, metabolic rate and body weight. A ribosomal toxin (saporin) conjugated to orexin-B was infused into the ventral tuberomammillary region of the hypothalamus, since most histaminergic neurons express orexin recep...

Background The integrity of the brain histaminergic system is necessary for the unfolding of homeostatic and cognitive processes through the recruitment of alternative circuits with distinct temporal patterns. We recently demonstrated that the fat-sensing lipid mediator oleoylethanolamide indirectly activates histaminergic neurons to exerts its hypophagic effects. The present experiments invest...

Different brain areas seem to be involved in the cardiovascular responses to stress. The medial amygdala (MeA) has been shown to participate in cardiovascular control, and acute stress activates the MeA to a greater extent than any of the other amygdaloid structures. It has been demonstrated that the brain histaminergic system may be involved in behavioral, autonomic and neuroendocrine response...

Circadian clocks allow anticipation of daily environmental changes. The suprachiasmatic nucleus (SCN) houses the master clock, but clocks are also widely expressed elsewhere in the body. Although some peripheral clocks have established roles, it is unclear what local brain clocks do. We tested the contribution of one putative local clock in mouse histaminergic neurons in the tuberomamillary nuc...

Possible central serotonergic and histaminergic modulation of acute peripheral inflammation was investigated in rats, adopting the formaldehyde-induced acute pedal inflammation as an experimental model. Intracerebroventricular (icv) administration of central inhibitory neurotransmitter, serotonin and its precursor, 5-hydroxytryptophan (5-HTP) attenuated the oedema volume and exudate protein con...

Background: The mechanisms of emergence from general anesthesia remain to be elucidated. Recent studies indicate that the central histaminergic system plays a critical role in maintaining wakefulness. In addition, neural pathways regulate wake-sleep cycle are involved anesthesia. this study, we determined propofol using microinjections and single-unit recordings rats.

Organotypic cultures of histaminergic tuberomammillary (TM) neurons were grown using explants obtained from newborn rats. The cultures were examined after immunohistochemical localization of the histamine synthetic enzyme, L-histidine decarboxylase (HDC). The morphological properties of the somata, dendrites and axons of HDC-immunoreactive TM neurons in organotypic culture were virtually indist...