The effect of halothane on the half-life of pentobarbitone was studied in sheep. Pentobarbitone was given in a dose of 20 mg/kg and halothane was administered via a mask for periods from 30 min up to 2 hr. Concentrations of pentobarbitone in whole Hood were measured and the half-life determined. In cross-over tests giving pentobarbitone alone, and pentobarbitone and halothane to eight sheep, in...

The volatile anesthetic halothane (2-bromo-2-chloro-1,1,1-trifluoroethane) is extensively metabolized in humans, with approximately 50% of an absorbed dose undergoing hepatic biotransformation (Carpenter et al., 1986). Halothane is a unique substrate, undergoing both oxidative and reductive P450-catalyzed dehalogenation during clinical anesthesia, with each metabolic pathway subtending a differ...

The volatile anesthetic halothane (2-bromo-2-chloro-1,1,1-trifluoroethane) is extensively metabolized in humans, with approximately 50% of an absorbed dose undergoing hepatic biotransformation (Carpenter et al., 1986). Halothane is a unique substrate, undergoing both oxidative and reductive P450-catalyzed dehalogenation during clinical anesthesia, with each metabolic pathway subtending a differ...

Previous investigations have shown that patients with fulminant hepatic failure after halothane anaesthesia have a circulating antibody which reacts with an antigen present on the surface of halothane-altered hepatocytes. In the present study, it has been shown that the expression of the antigen is associated with the oxidative metabolism of halothane, in contrast with results of other groups w...

We have studied the molecular mechanism of Ca-ATPase activation in sarcoplasmic reticulum (SR) by the volatile anesthetic halothane. Using time-resolved phosphorescence anisotropy, we determined the rotational correlation times and mole fractions of different oligomeric states of the enzyme, as a function of halothane and temperature. Lipid fluidity was measured independently, using EPR of spin...

We have investigated the effect of volatile anaesthetics on protein synthesis and secretion in Hartley male guinea pig liver slices. The slices (250-300 microns thick) were incubated in sealed roller vials containing Krebs-Henseleit buffer at 37 degrees C under 95% oxygen. Volatile anaesthetics were vaporized in the vials to produce constant concentrations in the medium. Halothane 1-2.1 mmol li...

We studied the electrical properties of intact muscle fibers from normal and malignant hyperthermia-susceptible (MHS) pigs. Resting membrane potentials, action potentials, and current-voltage relationships were measured with and without the presence of halothane. There were no changes in the resting potentials or the specific membrane conductances at any concentration of halothane in either the...

The effect of halothane on the amplitude and conduction time of the evoked nerve action potential of a mammalian nerve preparation was determined in vitro. The action of 4 per cent v/v halothane on the action potential was measured at three different hydrogen ion concentrations in the Krebs perfusate, namely, pH 6.57, pH 7.40, pH 8.00. Halothane potency, assessed by the depression of the action...

HALOGENATED anesthetic agents have been associated with perioperative liver dysfunction and injury. Most cases of hepatotoxicity reported involve halothane exposure. Halothane produces two types of hepatotoxicity. A mild, transient syndrome with modest increases in transaminase enzymes occurs in 20% of patients given halothane. A more severe and fulminant hepatitis with marked liver dysfunction...