BACKGROUND Recently, in order to improve the resistance of flax plants to pathogen infection, transgenic flax that overproduces β-1,3-glucanase was created. β-1,3-glucanase is a PR protein that hydrolyses the β-glucans, which are a major component of the cell wall in many groups of fungi. For this study, we used fourth-generation field-cultivated plants of the Fusarium -resistant transgenic lin...

The aim of this study was to analyze the major influence factors culture medium on expression level ?-1,3-1,4-glucanase, and further develop an optimized process for extracellular production ?-glucanase at a bioreactor scale (7 L) with genetically engineered Escherichia coli (E. coli) JM109-pLF3. In study, batch cultivation fed-batch including constant rate feeding strategy DO-stat (DO: Dissolv...

The ability of Bacillus subtilis, strain ALICA to produce three mycolytic enzymes (chitinase, β-1,3-glucanase, and protease), was carried out by the chemical standard methods. Bacillus subtilis ALICA was screened based on their antifungal activity in dual plate assay and cell-free culture filtrate (25%) against five different phytopathogenic fungi Alternaria alternata, Macrophomina sp., Colleto...

Cellulase production by a Cellulomonas sp., isolated in 1985 from forest humus soil along the border of the Caspian Sea in Iran, was investigated. This strain secreted endo-and exo-cellulases in the culture medium, but some of the enzymes produced remained cell membrane bound. Cell bound enzymes were released by various treatments. The highest amount of endo-glucanase (up to 95%) and exo-gl...

Fungal cell wall degrading chitinases and glucanases attained significance in agriculture, medicine, and environment management. The present study was conducted to describe the optimum conditions required for the production of beta-1,4-N-acetyl glucosaminidase (NAGase) and beta-1,3-glucanase by a biocontrol strain of Bacillus subtilis AF 1. B. subtilis AF 1 was grown in minimal medium with coll...

The objective of this study was to partially purify ß-glucanase from crude enzyme extract of straw mushroom (Volvariella volvacea). The crude enzyme extract was prepared from the fruiting bodies of straw mushroom. The ßglucanase was partially purified using an aqueous two-phase extraction with Triton X-114 and followed by ammonium sulfate precipitation in the range of 50-80% saturation. The ß-g...

We reported earlier that ethylene increased the levels of glucanase (endo-/3-1, 3-glucan-3-glucanohydrolase, EC 3 .2. 1 .6) in bean (Phaseolus vulgaris L. cv. Red Kidney) leaves (1, 2) at Frederick, Maryland. While the function for glucanase has not been firmly established, a number of roles have been proposed and include digestion of storage glucans in seeds, control of cell elongation, regula...

Lysobacter enzymogenes strain N4-7 produces multiple biochemically distinct extracellular -1,3-glucanase activities. The gluA, gluB, and gluC genes, encoding enzymes with -1,3-glucanase activity, were identified by a reverse-genetics approach following internal amino acid sequence determination of -1,3-glucanase-active proteins partially purified from culture filtrates of strain N4-7. Analysis ...

Clostridium thermocellum produces one major beta-1,3-glucanase. Genomic DNA fragments containing the gene were cloned from two strains, DSM1237(T) (6848 bp) and F7 (9766 bp). Overlapping sequences were 99.9 % identical. The nucleotide sequences contained reading frames for a putative transposase, endo-beta-1,3-1,4-glucanase CelC, a putative transcription regulator of the LacI type, beta-1,3-glu...

Cellulosimicrobium cellulans (also known with the synonyms Cellulomonas cellulans, Oerskovia xanthineolytica, and Arthrobacter luteus) is an actinomycete that excretes yeast cell wall lytic enzyme complexes containing endo-beta-1,3-glucanases [EC 3.2.1.39 and 3.2.1.6] as key constituents. Three genes encoding endo-beta-1,3-glucanases from two C. cellulans strains have been cloned and characteri...