Recommended Citation Tymkiw, Keelen D.. "The influence of smoking on cytokines in the gingival crevicular fluid in patients with periodontal disease." MS

Introduction Gingival crevicular fluid is exuded from the surrounding tissue of periodontal pockets in response to the pathogenic process in periodontitis. Gingival crevicular fluid contains microbial plaque, host inflammatory cells, host tissue and serumderived factors. As periodontal disease progresses, the volume of GCF increases and inflammatory cytokines appear in the GCF. GCF can be easil...

Background. Several studies in the last decades have focused on finding a precise method for the diagnosis of periodontal disease in its early stages. Aim. To evaluate from current scientific literature the most common and precise method for gingival crevicular fluid (GCF) sample collection, biomarker analytical methods, and the variability of biomarker quantification, even when using the same ...

The aim of this study was to determine the myeloid-related protein-8 and myeloid-related protein-14 levels in the gingival crevicular fluid of smoker patients with generalized aggressive periodontitis (SAgP), smoker patients with chronic periodontitis (SCP), smoker patients with gingivitis (SG-smoker control), non-smoker patients with generalized aggressive periodontitis (AgP), non-smoker patie...

BACKGROUND Azithromycin (AZM) is used in periodontal infections. The present study compared gingival crevicular fluid concentration of azithromycin of two pharmaceutical companies through the HPLC method. METHODS Two groups (n=15) of healthy volunteers participated in this study. The first group received an imported azithromycin (ImAZM) tablet (250 mg, PO) and the second group received an azi...

BACKGROUND Several studies have suggested an association between periodontal disease and prematurity but this finding has not been consistently observed. METHODS Case control study. Cases (n = 50) were women who had delivered after spontaneous preterm labor at <35 weeks gestation. Two groups of controls (n = 101) were recruited: women who were undelivered but at a preterm gestation and women ...

OBJECTIVE The aim of this study is to determine a protocol of gingival crevicular fluid protein extraction used for the first dimension of 2-DE gels. It also aims at conducting a review on the current candidates for protein markers of this pathology, all of which may be used to prevent the disease. METHODS Gingival crevicular fluid was collected from two groups of 60 patients each, with and w...

GCF is a serum transudate or inflammatory exudate that derived from the periodontal tissues and can be collected at orifice within gingival crevice.(1) The potential diagnostic importance of fluid was recognized more than 60 years ago serious investigation dynamics production did not begin until late 1950s with reports Brill co-workers.(2,3) component derives primarily microvascular leakage. Ad...

Modern research confirms the role of inflammatory mediators in the pathomechanism of periodontal tissue destruction. The aim of the study was to determine concentrations of MMP-8 and IL-1β in gingival crevicular fluid (GCF) in patients with advanced chronic and aggressive periodontitis. The authors measured the concentrations of the above inflammatory mediators in gingival crevicular fluid of d...

Micro-Raman Spectroscopy is an efficient method for analyzing biological specimens due to its sensitivity to subtle chemical and structural changes. The aim of this study was to use micro-Raman spectroscopy to analyze chemical and structural changes in periodontal ligament after orthodontic force application and in gingival crevicular fluid in presence of periodontal disease. The biopsy of peri...