Induction of protein synthesis by the external delivery of in-vitro transcription-messenger RNA (IVT-mRNA) has been a useful approach in the realm of cell biology, disease treatment, ‎reprogramming of cells, and vaccine design. Therefore, the development of new formulations for ‎protection of mRNA against nucleases is required to maintain its activity in-vivo. It was the aim o...

Background: Due to oxidative stress, hypoxia, and serum deprivation, a large percentage of mesenchymal stem cells (MSCs) die in the early stages of transplantation. The present study aimed to address whether induction or inhibition of autophagy would affect the viability of MSCs after exposure to oxidative stress.Methods: MSCs were isolated from umbilical cord tissue using the Ficoll grad...

background: the rapid increase of hiv-1 strains resistant to current antiretroviral drugs is a challenge for successful aids therapy. this necessitates the development of novel drugs, and to this end, availability of screening systems for in vitro drug discovery is a priority. herein, we report the modification of a previously developed system for increased sensitivity, ease of use, and cost-ef...

In the past decades, many studies have confirmed that specific personality traits correlate such a general factor of (GFP) emerges. order to test whether characteristics this are universal, thorough tests in various cultures required. Accordingly, we conducted meta-analysis on GFP mainland China. After testing sensitivity GFP, correction for attenuation, samples' geographical distribution, type...

La Proteína Verde Fluorescente (Green Fluorescent Protein, GFP) es ampliamente utilizada en ensayos in vivo e vitro. Se han generado múltiples variantes de esta proteína para diversificar sus características, como la GFP-enhancer (EGFP) que emite una señal fluorescencia 35 veces mayor comparación con silvestre, siendo implementada fusión estudios localización y estabilidad estructural, entre ot...

The scale invariance of the universe is slightly broken by slow roll parameters. It likely dual to random walk. We investigate distribution function conformal zeromode. identify de Sitter entropy $S_{dS}$ with zeromode $\rho(\omega)$. have collected convincing support on our postulate. semiclassical evidence that both are given gravitational coupling $1/g=\log N/2$ where $g=G_NH^2/\pi$ and $N$ ...

The gluten-free pasta (GFP) has a higher cost and is often brittle, pale, poorly balanced in macro micronutrients when compared to wheat-based pasta. This study aimed use the biofortified sweet potato flour, natural source of color provitamin A ingredient, with rice hydrolyzed soy protein concentrate, carboxymethyl cellulose gum produce GFP, using central composite rotatable design three factor...

Plant viruses classified in the genus Waikavirus , family Secoviridae are positive sense single-stranded RNA that include important pathogens of maize (maize chlorotic dwarf virus; MCDV) and rice (rice tungro spherical RTSV). Many aspects molecular biology waikaviruses remain unexplored because experimental challenges lack infectious clones for low titer, phloem-limited, obligately vector-trans...