نتایج جستجو برای: fungal dna

تعداد نتایج: 556901  

Journal: :Journal of clinical microbiology 1999
J Loeffler H Hebart R Bialek L Hagmeyer D Schmidt F P Serey M Hartmann J Eucker H Einsele

Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed. The identities of all contaminants were specified by cycle sequencing and GenBank analysis. Twelve of 150 PCR assays that...

تقی‌زاده ارمکی, مجتبی , شکوهی, طاهره , عسگری, محمد رضا , موذنی, مریم , نبیلی, مجتبی , نصرتی, احمد ,

Human fungal diseases are largely a 20th and 21st century’s phenomenon. Due to use of corticosteroids and antibacterial drug, medical developmenta are associated with increased risk for number of fungal disease. These nosocomial developments in invasive mycosis were paralleled over the last two decades by the human immunodeficiency virus (HIV) pandemic, which has resulted in an even larger numb...

Journal: :Environmental microbiology 2007
Stephan J Ott Nour Eddine El Mokhtari Ateequr Rehman Philip Rosenstiel Stephan Hellmig Tanja Kühbacher Markus Lins Rüdiger Simon Stefan Schreiber

Bacterial DNA has been found in coronary plaques and it has therefore been concluded that bacteria may play a role as trigger factors in the chronic inflammatory process underlying coronary atherosclerosis. However, the microbial spectrum is complex and it is not known whether microorganisms other than bacteria are involved in coronary disease. Fungal 18S rDNA signatures were systematically inv...

2014
Michiel Op De Beeck Bart Lievens Pieter Busschaert Stéphan Declerck Jaco Vangronsveld Jan V. Colpaert

Current metabarcoding studies aiming to characterize microbial communities generally rely on the amplification and sequencing of relatively short DNA regions. For fungi, the internal transcribed spacer (ITS) region in the ribosomal RNA (rRNA) operon has been accepted as the formal fungal barcode. Despite an increasing number of fungal metabarcoding studies, the amplification efficiency of prime...

Abdol satar Pagheh, Aynaz Ghojoghi, Mahdi Abastabar, Maryam Roudbary, Mehraban Falahati, Saham Ansari, Shirin Farahyar, Zeinab Ghasemi,

Background: Dermatophytes are the most common fungal agents causing superficial skin infections in worldwide. Species identification of these fungi is important for therapeutic and epidemiological apects. The purpose of this study was identification and epidemiology of dermatophytosis in patients referring to medical mycology laboratory of Razi hospital in Tehran, during 2014. Materials and Me...

Journal: :GSC biological and pharmaceutical sciences 2023

Medicinal plant possessed antifungal effects by many mechanisms, they caused membrane disturbance resulting in the loss of integrity, inhibited DNA transcription and reduced cell populations, activity fungal antioxidant enzymes biofilm formation. The current review discussed medicinal plants.

Journal: :Fungal Biology Reviews 2022

Intragenomic variation is the molecular within genome among repetitive DNA. As a multigene family, nuclear ribosomal DNA (rDNA) has been widely used in fungal taxonomy for their ease amplification and suitable variability to attain various levels of taxonomic resolution. At intraspecific level, rDNA believed be under concerted evolution internal transcribed spacers (ITS) region actually accepte...

Journal: :Journal of clinical microbiology 2006
Aihua Huang Jun-Wen Li Zhi-Qiang Shen Xin-Wei Wang Min Jin

Here we report the development of an oligonucleotide microarray method that can identify fungal pathogens in a single reaction. Specific oligonucleotide probes targeted to internal transcribed spacer 2 were designed and synthesized. Fungal DNA was amplified by universal primers, and the PCR product was hybridized with the oligonucleotide microarray. A series of specific hybridization profiles c...

Journal: :Letters in applied microbiology 2010
Y J Zhang S Zhang X Z Liu H A Wen M Wang

AIMS A simple and rapid method (designated thermolysis) for extracting genomic DNA from bulk fungal strains was described. METHODS AND RESULTS In the thermolysis method, a few mycelia or yeast cells were first rinsed with pure water to remove potential PCR inhibitors and then incubated in a lysis buffer at 85 degrees C to break down cell walls and membranes. This method was used to extract ge...

Journal: :Indian journal of experimental biology 2014
Divya Jose P Jayesh Prem Gopinath A Mohandas I S Bright Singh

Pseudomonas aeruginosa MCCB 123 was grown in a synthetic medium for beta-1,3 glucanase production. From the culture filtrate, beta-1,3 glucanase was purified with a molecular mass of 45 kDa. The enzyme was a metallozyme as its beta-1,3 glucanase activity got inhibited by the metal chelator EDTA. Optimum pH and temperature for beta-1,3 glucanase activity on laminarin was found to be 7 and 50 deg...

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