Two different classes of fluorescent dyes were prepared as a turn off/on sensor system for aldehydes. Amino derivatives of a boron dipyrromethene (BDP) fluorophore and a xanthene-derived fluorophore (rosamine) were prepared. Model compounds of their product with an aldehyde were prepared using salicylaldehyde. Both amino boron dipyrromethene and rosamine derivatives are almost non-fluorescent i...

The paper reports on the synthesis and fluorescence characteristics of novel 1,8-naphthalimide fluorophore. It was configured as a “fluorophore-spacerreceptor” system able to act pH-probe via PET (photoinduced electron transfer) sensing mechanism. Due tertiary amine receptor probe showed “off-on” switching properties under transition from alkaline acid media. Also, ability detect metal ions in ...

Spinach is an in vitro-selected RNA aptamer that binds a GFP-like ligand and activates its green fluorescence. Spinach is thus an RNA analog of GFP and has potentially widespread applications for in vivo labeling and imaging. We used antibody-assisted crystallography to determine the structures of Spinach both with and without bound fluorophore at 2.2-Å and 2.4-Å resolution, respectively. Spina...

Because RNA lacks strong intrinsic fluorescence, it has proven challenging to track RNA molecules in real time. To address this problem and to allow the purification of fluorescently tagged RNA complexes, we have selected a high affinity RNA aptamer called RNA Mango. This aptamer binds a series of thiazole orange (fluorophore) derivatives with nanomolar affinity, while increasing fluorophore fl...

Fluorophore mediated bio-signal retrieval has been extensively used in molecular imaging. However, only a limited number of fluorophores can be used for humans and their quantum yield is usually low. Another important issue is emitting fluorescence at the disease site, with a minimal non-specific emission at any other sites. Artificial quenching and enhancing of fluorescence was found to be pos...

Differential accessibility of liganded, high affinity rabbit anti-fluorescyl IgG antibody combining sites to the aqueous milieu has been investigated by solvent perturbation of the extrinsic fluorescence of bound fluorophore. Iodide, a dynamic quencher of fluorescein, was selected for use in these studies after examination of a number of water-soluble fluorescence quenchers. Quenching of antibo...

The recent surge in interest in the metal-enhanced fluorescence MEF phenomenon and its numerous applications in the biosciences has fueled research into identifying alternative metals to silver which have desirable properties, such as enhanced emission and fluorophore photostability. In this paper, we subsequently study and reveal that tin nanodeposits are a suitable metal for MEF with an elect...

We present the synthesis and characterization of a family regioisomerically pure pH-sensitive rosamine fluorophores consisting xanthene fluorophore cores differently substituted phenol moieties.

A method for analyzing combinatorial DNA arrays using oligonucleotide-modified gold nanoparticle probes and a conventional flatbed scanner is described here. Labeling oligonucleotide targets with nanoparticle rather than fluorophore probes substantially alters the melting profiles of the targets from an array substrate. This difference permits the discrimination of an oligonucleotide sequence f...