Background: Extensive full-thickness burns require replacement of both epidermis and dermis. In designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. Methods: In the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chito...

Background: Human fibroblasts are the part of the dermis that secrete extracellular matrix for the purpose of tissue repair. Culturing fibroblasts, which leads to formation of a monolayer of these cells, is used for treating various conditions including thermal burns and other skin defects such as diabetic and varicose vein leg ulcers. Therefore, we aimed at developing a fibroblast bank to acco...

background and aim(s): this in vitro study compared the effects of mineral trioxide aggregate (mta), calcium enriched mixture (cem) cement, biodentine (bd) and octacalcium phosphate (ocp) on the viability of human gingival fibroblasts (hgfs). material and methods: after completion of the setting time of understudy materials, fibroblasts were placed in 24-well insert plates and 1mg of the unders...

the alteration of cytokine balance is stated to exert greater influence on gingival overgrowth compared to the direct effect of the drug on the regulation of extracellular matrix metabolism. the current study evaluated the effect of phenytoin on the regulation of collagen, lysyl oxidase and elastin in gingival fibroblasts.normal human gingival fibroblasts (hgfs) were obtained from 4 healthy chi...

in recent years transdifferentiation technology has enabled direct conversion of human fibroblasts to become a valuable, abundant and accessible cell source for patient-specific induced cell generation in biomedical research. the majority of transdifferentiation approaches rely upon viral gene delivery which due to random integration with the host genome can cause genome instability and tumorig...

In this study, we have investigated the proliferative response of human dermal fibroblasts isolated from normal and psoriatic individuals to different cytokines. We found that IL-1, TNF (α) and EGF are stimulatory whereas IFN « and 1 both showed inhibitory activity on fibroblast proliferation. IL-6 a more recently described cytokine, showed no detectable effects on cell proliferation. Inve...

Senescent cells contribute to the functional decline of skin via senescence-associated secretory phenotypes. The accumulation senescent plays a role in development photoaging pigmentation and aging. We investigated age-dependent cellular senescence vivo skin. Seventy biopsied skins taken from 1 81 years old subjects were obtained. samples grouped age 0-20, 21-40, 41-60 61-81 further classified ...

background and aims. since intracanal medicaments can affect the cell viability in periapical tissues, the aim of this study was to evaluate the effect of calcium hydroxide and propolis on pulp fibroblasts. materials and methods. two healthy third molars were used as a source to obtain fibroblasts. the fibroblasts were cultured and subjected to 1 mg/ml of propolis and calcium hydroxide. this ex...