نتایج جستجو برای: feeder layer

تعداد نتایج: 287296  

Journal: :ACS applied materials & interfaces 2013
Yongbiao Zhao Jiangshan Chen Dongge Ma

In this paper, highly efficient and simple monochrome blue, green, orange, and red organic light emitting diodes (OLEDs) based on ultrathin nondoped emissive layers (EMLs) have been reported. The ultrathin nondoped EML was constructed by introducing a 0.1 nm thin layer of pure phosphorescent dyes between a hole transporting layer and an electron transporting layer. The maximum external quantum ...

Journal: :Tissue engineering 2007
Federica Riva Andrea Casasco Emanuele Nespoli Antonia Icaro Cornaglia Marco Casasco Angela Faga Silvia Scevola Giuliano Mazzini Alberto Calligaro

Because engineered tissues are designed for clinical applications in humans, a major problem is the contamination of cocultures and tissues by allogenic molecules used to grow stem cells in vitro. The protocols that are commonly applied to generate epidermal equivalents in vitro require the use of irradiated murine fibroblasts as a feeder layer for keratinocytes. In this study, we report a simp...

Journal: :Journal of biomechanical engineering 2008
Liang Zhu Rupak K Banerjee Maher Salloum Albert Bachmann Robert W Flower

Laser photocoagulation of the feeder vessels of age-related macula degeneration-related choroidal neovascularization (CNV) membranes is a compelling treatment modality, one important reason being that the treatment site is removed from the fovea in cases of sub- or juxtafoveal CNV. To enhance the energy absorption in a target feeder vessel, an indocyanine green dye bolus is injected intravenous...

Journal: :مجله دانشگاه علوم پزشکی شهید صدوقی یزد 0
مرتضی یافتیان m yaftiyan مژده صالح نیا m salehnia علی اکبر پور فتح اله aa pourfatollah

introduction: considering the importance of co-culture in differentiation of embryonic stem cells, the aim of this study was evaluation of the effect of co-culturing fetal liver stroma cells with p19 cells on the line of differentiation. materials and methods: for this purpose, p19 cells were cultured directly in semisolid medium. these cells proliferated and primarily differentiated to colonie...

Journal: :Iranian biomedical journal 2009
Hassan Hashemi Mojdeh Salehnia Morvarid Kamali Mandana Beigi Boroujeni

BACKGROUND This study was undertaken to establish the characterization of cultured oral mucosal epithelium and introducing them as an alternative source for reconstruction of ocular surface disease. METHODS Human oral epithelial cells were cultured on simple media (DMEM/HF12) as control and co-cultured on mitomycin C-treated 3T3 feeder layer, on the amniotic membrane (AM) without nitrocellulo...

2014
Shan Cong Guifang Cao Dongjun Liu

To find a suitable feeder layer is important for successful culture conditions of bovine embryonic stem cell-like cells. In this study, expression of pluripotency-related genes OCT4, SOX2 and NANOG in bovine embryonic stem cell-like cells on mouse embryonic fibroblast feeder layers at 1-5 passages were monitored in order to identify the possible reason that bovine embryonic stem cell-like cells...

صالح نیا, مژده, پور فتح اله, علی اکبر, یافتیان, مرتضی,

Introduction: Considering the importance of co-culture in differentiation of embryonic stem cells, the aim of this study was evaluation of the effect of co-culturing fetal liver stroma cells with P19 cells on the line of differentiation. Materials and Methods: For this purpose, P19 cells were cultured directly in semisolid medium. These cells proliferated and primarily differentiated to col...

ژورنال: پیاورد سلامت 2012
آبرون, سعید, حاجی فتحعلی, عباس, خمیسی پور, غلامرضا, ذنوبی, زهرا, سلیمانی, مسعود, صوفی زمرد, مینا, مصاحبی محمدی, مجید, کاویانی, سعید,

Background and Aim: Embryonic stem cells are identified with two unique characteristics. First, they can be maintained and expanded as pure populations of undifferentiated cells, a characteristic which is known as self renewal aspect of embryonic stem cells. Second, these cells can give rise to all body cell types. In the current study, we used a feeder-free condition to differentiate mouse emb...

Journal: :iranian journal of basic medical sciences 0
nahid ataie nejad department of reproductive biology, faculty of medicine, tehran university of medical sciences, tehran, iran fardin amidi department of anatomical sciences, faculty of medicine, tehran university of medical sciences, tehran, iran marziyeh agha hoseini ivf center, shariati hospital, tehran university of medical sciences, tehran, iran karim nayer nia institute for molecular medicine and cell therapy, düsseldorf, germany and geneocell, advanced molecular & cellular technologies, tehran, iran mehryar habibi blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran abdol mohammad kajbafzadeh pediatric urology research center, children hospital medical center, tehran university of medical sciences, tehran, iran

objective(s):mesenchymal stem cells (mscs) derived from wharton’s jelly (wj-mscs) are now much more appealing for cell-based infertility therapy. hence, wj-mscs differentiation toward germ layer cells for cell therapy purposes is currently under intensive study. materials and methods: mscs were isolated from human wharton’s jelly and treated with bmp4, retinoic acid (ra) or co-cultured on human...

2013
Jingjing Ma Ling Tian Jin Cheng Zhiwei Chen Bing Xu Liwei Wang Chuanyuan Li Qian Huang

Tumor growth after radiotherapy is a commonly recognized cause of therapeutic failure. In this way, we examined tumor cell growth after radiotherapy by establishing a cancer cell growth model in vitro. We accomplished this model by seeding non-irradiated firefly luciferase2 and green fluorescent protein fusion gene (Fluc) labeled living cancer reporter cells onto a feeder layer of irradiated ca...

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