An efficient protocol providing a dual regeneration pathway via direct shoot organogenesis and somatic embryogenesis for an endangered species, Metabriggsia ovalifolia W. T. Wang, was established from leaf explants. When applied at 2.5 μM, the cytokinins 6-benzyladenine (BA) or thidiazuron (TDZ) and the auxins indole-3-butyric acid (IBA), α-naphthaleneacetic acid (NAA) and indole-3-acetic acid ...

In order to select the best tomato cultivar for Agrobacterium mediated gene transformation studies, In vitro regeneration frequency of hypocotyls, leaf disc and shoot tip of five tomato cultivars (Lycopersicon esculentum) was investigated on a regeneration medium supplemented with 1 mg/l zeatin and 0.1 mg/l indole-3-acetic acid. Significant differences in regeneration capacity between genotypes...

cumin (cuminum cyminum l.) as a member of the apiaceae family is one of the most important medicinal plants in iran. the purpose of this study was to evaluate the effect of plant growth regulators and explant type on callus induction in cumin. for this purpose the cumin seeds (kuhbanan accession) were disinfected with sodium hypochlorite and alcohol and cultured on ms basal medium. leaf and hyp...

The 'fishmouth phenomenon' seen in some previously treated retinal detachments is associated with large horeshoe tears and scleral buckling techniques. A method of treating patients with this complication is described in which scleral buckling with implant or explant is not used. The technique utilises intravitreal air tamponade with cryopexy and gives good results. The incidence of patients de...

BACKGROUND Explant browning presents a major problem for in vitro culture, and can lead to the death of the explant and failure of regeneration. Considerable work has examined the physiological mechanisms underlying Phalaenopsis leaf explant browning, but the molecular mechanisms of browning remain elusive. In this study, we used whole genome RNA sequencing to examine Phalaenopsis leaf explant ...

The hypothalamic neuropeptide melanin-concentrating hormone (MCH) increases feeding when injected intracerebroventricularly in rats. To identify the hypothalamic nuclei responsible for the orexigenic effect, we injected the peptide into discrete hypothalamic nuclei known to express the MCH receptor, MCH1R. MCH (0.6 nmol) elicited a rapid and significant increase in feeding in satiated rats foll...