The aim of the present study was to find out role Ruk/CIN85 in modulation activities/content key enzymes/components glycolysis and hydrogen peroxide using as a model human NSCLC MOR wild type resistant drugs MOR/0.2R cells. Materials methods. (ECACC 84112312) 96042335), drug-resistant cell line, were cultured complete RPMI medium under standard conditions. Enzymes activity, content metabolites ...

Steady-state enzyme kinetics is a cornerstone technique of biochemistry and related sciences since it allows the characterization quantification behaviour. Enzyme widely used to investigate physiological role enzymes, determine effects mutations characterize inhibitors. Well-known examples inhibitors treat diseases include anti-infectives (e.g., penicillin, clavulanic acid HIV protease inhibito...

We investigated the role of 1-deoxynojirimycin (DNJ) on glucose absorption and metabolism in normal and diabetic mice. Oral and intravenous glucose tolerance tests and labeled (13)C6-glucose uptake assays suggested that DNJ inhibited intestinal glucose absorption in intestine. We also showed that DNJ down-regulated intestinal SGLT1, Na(+)/K(+)-ATP and GLUT2 mRNA and protein expression. Pretreat...

angiogenesis or formation of new blood vessels from preexisting vasculature is a key process in some physiological conditions such as wound healing, growth, and action of female reproductive organs. moreover, disturbance of the mechanisms of physiological angiogenesis has a role in pathogenesis of some diseases in the form of overproliferation of blood vessels such as cancers, psoriasis, arthri...

Background: Probiotics have attracted a great attention aiming to develop natural non-toxic antioxidants, because of their role in decreasing the risk of reactive oxygen species (ROS) accumulation. Objectives: The purpose of this study was to assess the antioxidant activity of a probiotic Streptococcus salivarius ssp thermophillus (St.sa) and to evaluate its protective effect against the ...

Twenty isolates of Trichoderma harzianum wereisolated from the rhizosphere of healthy pistachio plants from different localities of Kerman Province, Iran.Five isolates with high antagonistic activity in in vitro assays against Verticillium dahliae (the causal agent of pistachio wilt), were investigated for their effect on the defense enzymes, peroxidase (PO), phenyl alanine-ammonia lyase (PAL) ...

UDP-glucuronosyltransferases (UGTs), located in the endoplasmic reticulum of liver cells, are an important family enzymes, responsible for biotransformation several endogenous and exogenous chemicals, including therapeutic drugs. However, phenomenon 'latency', i.e., full UGT activity revealed by disruption microsomal membrane, poses substantial challenges predicting drug clearance based on vitr...

Enzymes that act on DNA are promising targets for anticancer therapeutics1. For example, the epigenetic enzyme DNMT1 is inhibited by 5-azacytidine, causing DNA hypomethylation and enhancing the function of tumor suppressor genes2. In concordance, the development of accurate and user-friendly assays to study DNA modifications has become pivotal to drug discovery. Traditional in vitro assays have...

We have developed high-throughput microtitre plate-based assays for DNA gyrase and other DNA topoisomerases. These assays exploit the fact that negatively supercoiled plasmids form intermolecular triplexes more efficiently than when they are relaxed. Two assays are presented, one using capture of a plasmid containing a single triplex-forming sequence by an oligonucleotide tethered to the surfac...