Three methods were compared for the detection of prunus necrotic ringspot virus in herbaceous and woody plants: DAS-ELISA, nonisotopic dot-blot hybridization and reverse transcriptional polymerase chain reaction (RT-PCR). When purified virus preparations were used, the detection limit of the RT-PCR technique was 1·28 pg mL whereas nonisotopic molecular hybridization and DAS-ELISA allowed detect...

In recent years, the symptoms of viral diseases such as dwarfism, mosaic, discoloration, necrosis, and circular spots have been prevalent in ornamental plants in parks, gardens, and streets in North Khorasan Province. Tomato ring spot virus (ToRSV) belongs to the Nepovirus genus of Secoviridae family. This virus is a major cause of the infection of ornamental plants in the world. In this study,...

The relative functional and/or structural importance of different amino acid sites in a protein can be assessed by evaluating the selective constraints to which they have been subjected during the course of evolution. Here we explore such constraints at the linear and three-dimensional levels for the movement protein (MP) and coat protein (CP) encoded by RNA 3 of prunus necrotic ringspot ilarvi...

A new tobamovirus species has been recently isolated from hibiscus (Hibiscus rosa-sinensis) in Florida (1). This Florida hibiscus virus has been given the name Hibiscus latent Fort Pierce virus (HLFPV) to reflect the site (Fort Pierce, FL) and host (hibiscus) from which it was originally isolated. A limited survey has revealed that this virus is widespread in hibiscus and related species in the...