نتایج جستجو برای: drug assay

تعداد نتایج: 784710  

2006
James Carmichael William G. DeGraff Adi F. Gazdar John D. Minna James B. Mitchell

Drug sensitivity assays were performed using a variation of a colori metrie [3-{4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)1 assay on V79, CHO-AuxBl, CH"C5, NCI-H460, and NCIH249 cell lines following optimization of experimental conditions for each cell line. Results from this assay were compared with data assimi lated simultaneously by clonogenic assay and by dye exclusion ...

2013
Noh Jin Park Xiuqiang Wang Angelica Diaz Dana M. Goos-Root Christopher Bock Jonathan D. Vaught Weimin Sun Charles M. Strom

BACKGROUND Response to cetuximab (Erbitux®) and panitumumab (Vectibix®) varies among individuals, and even those who show response ultimately gain drug resistance. One possible etiologic factor is differential interaction between the drug and target. We describe the development of an assay based on Slow Off-rate Modified Aptamer (SOMAmer(™)) reagents that can distinguish drug-bound from unbound...

1987
James Carmichael William G. DeGraff Adi F. Gazdar John D. Minna James B. Mitchell

Drug sensitivity assays were performed using a variation of a colori metrie [3-{4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)1 assay on V79, CHO-AuxBl, CH"C5, NCI-H460, and NCIH249 cell lines following optimization of experimental conditions for each cell line. Results from this assay were compared with data assimi lated simultaneously by clonogenic assay and by dye exclusion ...

Journal: :Journal of Pure and Applied Microbiology 2022

Non-tuberculous Mycobacteria (NTM) are emerging as an important opportunistic pathogen. Since clinical presentation of NTM infection is similar to tuberculosis (TB), patients present suspected TB or drug resistant TB. Presently in the National Tuberculosis Elimination Programme (NTEP ) not being speciated, but there urgent need characterize so that appropriate treatment can be given many specie...

Journal: :research in pharmaceutical sciences 0

alpha-1antitrypsin (a1at) deficiency, an inherited disorder, has been shown to be the cause of lung diseases such as emphysema and chronic obstructive pulmonary disease. one of the treatment strategies to provide appropriate and adequate concentrations of a1at in the lungsis the application of nanoparticles (nps) in pulmonary drug delivery. in the current study, biocompatible nanohydrogels were...

Journal: :Antimicrobial agents and chemotherapy 2009
Edgie-Mark A Co Richard A Dennull Drew D Reinbold Norman C Waters Jacob D Johnson

Several drug development strategies, including optimization of new antimalarial drug combinations, have been used to counter malaria drug resistance. We evaluated the malaria Sybr green I-based fluorescence (MSF) assay for its use in in vitro drug combination sensitivity assays. Drug combinations of previously published synergistic (atovaquone and proguanil), indifferent (chloroquine and azithr...

Journal: :Cancer research 2012
Linda Bosserman Franklyn Prendergast Roy Herbst Martin Fleisher Emery Salom Steven Strickland Anastasios Raptis Allan Hallquist Mathieu Perree Swapnil Rajurkar Misagh Karimi Karl Rogers Dirk Davidson Carl Willis Manuel Penalver Howard Homesley Matthew Burrell Audrey Garrett James Rutledge Michael Chernick Cary A Presant

A drug-induced apoptosis assay, termed the microculture-kinetic (MiCK) assay, has been developed. Blinded clinical trials have shown higher response rates and longer survival in groups of patients with acute myelocytic leukemia and epithelial ovarian cancer who have been treated with drugs that show high apoptosis in the MiCK assay. Unblinded clinical trials in multiple tumor types have shown t...

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