Different experimental conditions have been compared to achieve a high efficiency in embryogenic calli initiation from 'Macabeo' and 'Tempranillo' anthers. Specifically, two stages of anther development were tested (corresponding to tetrad cells or uninucleate pollen), and direct culture of anthers was compared to culture after a cold treatment of inflorescences (4 °C during 48 h). In addition,...

Legumes are an alternative to animal foods due their high protein content. Like other legumes, Vicia faba (faba bean) has However, bean breeding with classical methods is challenging inbreeding depression, self-incompatibility, and abortive embryo formation. The inadequacy of the problems in fertilization biology plant limits production new varieties. Therefore, importance using vitro haploidiz...

History of Plant Tissue Culture and Biotechnology Scope and Importance of Biotechnology Tissue and Cell Culture Micropropagation Anther Culture Production of Secondary Metabolites Protoplasts Isolation and Fusion Somatic Hybrids Somaclonal Variation Germplasm Conservation Genetic Engineering and Gene Technology Methods for Gene Transfer Generation of Transgenic Plants and their identification M...

BACKGROUND AND AIMS Dioecism characterizes many crop species of economic value, including kiwifruit (Actinidia deliciosa). Kiwifruit male sterility occurs at the microspore stage. The cell walls of the microspores and the pollen of the male-sterile and male-fertile flowers, respectively, differ in glucose and galactose levels. In numerous plants, pollen formation involves normal functioning and...

Anther culture technique is one of the commonly used method in order to obtain haploid plants eggplant. It known that androgenic response eggplant variable and most important factors determining success genotype effect. Low or insufficient embryogenic limits use double technology breeding programs. Therefore, responses genotypes a step for this studies. In study, 61 at various stages (F1, F2, F...

CONTENTS History of Plant Tissue Culture and Biotechnology Scope and Importance of Biotechnology Tissue and Cell Culture Micropropagation Anther Culture Production of Secondary Metabolites Protoplasts Isolation and Fusion Somatic Hybrids Somaclonal Variation Germplasm Conservation Genetic Engineering and Gene Technology Methods for Gene Transfer Generation of Transgenic Plants and their identif...