Chemicals All the oligonucleotides were purchased from Beijing AuGCT DNA-SYN Biotechnology Co. Ltd (Beijing, China). Exonuclease III (Exo III) along with the reaction buffer, 10 × NEBuffer 1 (100 mM bis-tris-propane-HCl, 100 mM MgCl 2 and 10.0 mM dithiothreitol, pH 7.0) and 10 × NEBuffer 3 (100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl 2 , 1 mM dithiothreitol pH 7.9) was purchased from New England Bi...

The following substances were added to a basic diluent for fowl semen either separately or in combination: caproic acid, formaldehyde, acetyl carnitine, adenine, inosine, sodium pyruvate, succinic acid, dithiothreitol, sodium citrate and zinc, chloride, bicarbonate and phosphate. No significant improvement in the fertility of semen stored at 5 degrees C for either 24 or 48 hrs was obtained over...

Analysis of polyoma virions by X-ray fluorometry demonstrated that calcium (Ca2+) was associated with the purified virion. Treatment of purified virions with ethyleneglycol-bis-N,N'-tetraacetic acid (EGTA), which chelates Ca2+, and the reducing agent dithiothreitol caused the virions to dissociate. Electron microscopy revealed that the virions were dissociated to the capsomere level. Incubation...

When intact Kalanchoë plants are illuminated NADP-linked malic dehydrogenase and three enzymes of the reductive pentose phosphate pathway, ribulose-5-phosphate kinase, NADP-linked glyceraldehyde-3-phosphate dehydrogenase, and sedoheptulose-1,7-diphosphate phosphatase, are activated. In crude extracts these enzymes are activated by dithiothreitol treatment. Light or dithiothreitol treatment does...