BACKGROUND AND AIMS Dendrobium longicornu, commonly known as the 'Long-horned Dendrobium', is an endangered and medicinally important epiphytic orchid. Over-exploitation and habitat destruction seriously threaten this orchid in Northeast India. Our objective was to develop an efficient protocol for the mass propagation of D. longicornu using axillary bud segments. METHODOLOGY AND PRINCIPAL RE...

Rice plant regeneration via anther culture possess several difficulties, these included early necrosis and high albinism frequency. In the present study, biotic abiotic factors were studied to develop an efficient protocol for of Malaysian indica rice MR 219 variety. Callus initiation cultures was evaluated using different N6 media supplemented with 2,4-D in combination 1-naphthaleneacetic acid...

Teucrium chamaedrys is regarded as an herbaceous perennial plant from Lamiaceae family that is used as medicinal plants and food from ancient times. Due to the recent developments in tissue culture techniques and extraction of secondary metabolites, this study aimed to investigate the explants of the Teucrium for callus induction and secondary metabolites. The interaction of Auxin hormones incl...

We have cloned a SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) gene from Medicago truncatula (MtSERK1) and examined its expression in culture using real time PCR. In the presence of the auxin 1-naphthaleneacetic acid (NAA) alone, root differentiation occurs from the proliferating calli in both the cultured highly embryogenic seed line (2HA) and a low to nonembryogenic seed line (M. truncatula cv...

An efficient protocol was developed for the regeneration of plantlets via direct and indirect organogenesis from stem nodal segments of Naravelia zeylanica (L.) DC. (Ranunculaceae). The interaction of 6-benzyl aminopurine (BAP) with -naphthalene acetic acid (NAA) in Murashige and Skoog (1962) medium induced a varied morphological response. A maximum of 17.6 ± 1.5 shoots per explant were obtaine...

A reliable regeneration system for faba bean has been difficult to establish and therefore, the genetic improvement of Vicia faba L. was delayed. The paper describes a method of somatic embryo induction in callus of V. faba. Two Egyptian faba bean cultivars 'Giza 2' and '24 Hyto' were used. Callus was induced from epicotyls and shoot tips cultured on MS or Gamborg medium supplemented with 3% su...