We devised a new in vitro method to estimate the proportion of dividing cells and the potential doubling time (Tpot) of tumors using the same technique as with the cytokinesis-block micronucleus assay. The usefulness of this methodology was confirmed by comparing the data with those obtained by flow cytometry after bromodeoxyuridine (BrdUrd) incorporation. Xenografted human and murine tumors we...

Dear Editor, I was very pleased to find that the article” Micronucleus Assay of Buccal Mucosa Cells in Waterpipe (Hookah) Smokers: A Cytologic Study “(1) has attracted the attention of some readers and has created a few points for them. In my opinion, these points are good bases to overcome some concerns regarding micronucleus assay.

background: stress may cause damages to dna or/and change the ability of the cells to overcome these damages. it may also cause irregularities in the cell cycle and induce abnormal cell divisions through glucocorticoid-dependent functions. the abnormal cell divisions, in turn, lead to chromosomal mal-segregation and aneuploidy. in this study, the effects of the stress hormone, hydrocortisone (h...

Hydroquinone (HQ) has been reported to produce chromosomal effects in some in vivo and in vitro animal models. Its potential for inducing similar effects in human lymphocytes is less clear. The purpose of this study was to examine human lymphocytes treated with HQ for the presence of chromosomal anomalies, using an accepted assay for micronuclei. In addition, the stability of HQ in culture medi...

The multi-endpoint cytokinesis-blocked micronucleus assay is used for assessing chromosome aberrations. We have recently reported that this assay is extremely sensitive to genetic damage caused by the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and that the binucleated cells with micronuclei, nucleoplasmic bridges, and nuclear buds in lymphocytes (chromosom...

crude oil is enriched in polycyclic aromatic hydrocarbons (pahs). many pah analogs have proved to potentially damage dna. dna damage can be assessed using various biomarkers to find out the degree of genotoxicity of pollutants following in vitro exposure. in this research the comet assay and micronucleus (mn) test were used to detect dna damages and cytogenetic changes following crude oil expos...

conclusions b. oleracea demonstrated similar anti-inflammatory properties to omeprazole in rats with gerd. however, omeprazole also demonstrated genotoxic and cytotoxic effects in rats without gerd. results rats with gerd developed esophagitis and esophageal squamous cell carcinoma. b. oleracea and omeprazole-treated gerd rats presented significantly decreased inflammation in relation to non-tr...

background: radio-adaptive response and bystander effects are known phenomena occurring in cells following exposure to ionizing radiation (ir). in this study we examined possible radio-adaptation of lymphocytes following bystander effects induced by cho-k1 cells. materials and methods: whole blood and cho-k1 cells were cultured in rpmi-1640 complete medium. cells were separately irradiated with...

These studies are concerned with "mapping" the temporal order of the precommitment events in the differentiation pathway of the Friend erythroleukemia cell. We have used a single-block procedure in which a differentiation-specific inhibitor of a temperature-sensitive (ts) differentiation-defective mutation was used to block the differentiation program. Later, the block was removed, differentiat...