Background: In contrast to animal data, group culture does not increase preimplantation development in humans. This prospective study was done to test a new type of culture dish allowing for individual culture and any possible effect of autotrophic factors. Materials and Methods: Within a 6-month period, 72patients with ≥9 fertilized eggs were enrolled in this prospective evaluation. Their 936 ...

Many previous studies have aimed at spermatogenesis of male murine germ cells in vitro, but no efficient system has been established yet that covers the entire process of mammalian spermatogenesis in a culture dish permanently. In this review, we report on the requirements of spermatogenesis and the current state of different culture methods using testicular tissue fragments, single cell suspen...

We have succeeded in corroborating the enhancing effect of vitamin A, vitamin C, sitosterol and fucosterol on the fibrinolytic activity of endothelial cells. The assay system consisted of an in situ dissolution of a fibrin layer coated onto a culture dish, over which endothelial cells were grown in a culture medium containing 10% serum. The dissolution was enhanced by the addition of these vita...

When he finished school, Fleming trained as a doctor. He liked experimenting with bacteria and germs and often grew cultures in unwashed, shallow, circular dishes. One day, Fleming accidentally made a big discovery that made him very famous. Fleming was going on holiday for two weeks. He left his London laboratory in a mess and left his window open by mistake. In his haste, he also forgot to cl...

Growth properties of cells cultured from primary mammary tumors of C3H mice have been analyzed. Cells were seeded at 2 different densities (1 X 10(5) and 5 X 10(5)/sq cm) and were supported with a culture fluid containing 10% fetal calf serum and 5 mug insulin per ml. Mitosis continued after confluence was achieved, but cells did not accumulated in the monolayer; rather, certain cells were rele...

Imogolite is a naturally occurring aluminosilicate clay mineral with a nanotube structure. In this study, synthetic imogolite nanotubes were applied to cell culture and the properties as scaffold were compared with those of a conventional culture dish and the carbon nanotube scaffold. The surface characteristics of the imogolite scaffold were drastically changed with the amount of imogolite on ...

Under appropriate culture conditions human embryonic stem cells (hESCs) can retain an undifferentiated state during numerous passages (Thomson et al., 1998). In the undifferentiated state, hESCs express characteristic markers like NANOG, OCT4, TDGF1, DNMT3B, GABRB3, and GDF3, and are maintained by plating undifferentiated cells or colonies of cells into new culture dishes with fresh medium ever...

Differences in the characteristics of the culture conditions can influence the multiplication rate of Plasmodium falciparum. The Petri dish method is one of the most popular methods of cultivating this parasite. In many previous studies, ideal culture conditions of the Petri dish method were achieved by using erythrocytes collected from blood that had been stored for at least 2 weeks, with dail...