Mares can be artificially inseminated with chilled or frozen semen to increase the revenue from their offspring. Embryo transfer can be used to produce more than one foal from a single mare per season. Recent advances in using equine follicle-stimulating hormone to induce superovulation in mares have stimulated research on preserving equine embryos. Equine embryos are usually collected on day 7...

This review summarises the most significant findings related to the issue of the quantitative and qualitative indicators of stallion ejaculates, its processing when producing chilled insemination doses and the possibility of influencing the individual steps of the entire process. Artificial insemination, with cooled-storage semen, is increasingly used in horse breeding because it offers many ad...

Modern livestock breeding is basically dependent on the proper use of semen for artificial insemination (AI) of females and of other reproductive biotechnologies such as the production of embryos in vitro for embryo transfer (IVP). Both these techniques have made possible not only the wide dissemination of genetic material onto breeding populations but also enhanced the selection of best sires,...

Introduction Both semen collection and artificial insemination (AI) in cats was first described more than 30 years ago [1] but the procedures are still not routine because very few veterinary clinics are offering these services. While the more advanced biotechnology techniques such as in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI) and cloning have been successfully perfor...

Spermatozoa, especially those of the porcine species, are highly susceptible to in vitro chilling and ageing. Extenders are continuously developed to protect boar spermatozoa from chilling injury. New semen extenders and other modified preservation strategies require sensitive testing for essential sperm functions. The key process on the pathway of fertilization is capacitation. The aim of the ...

Artificial insemination (AI) using chilled ram semen has not been adopted in Uruguay yet, probably due to a lack of information about the use of this technology in the country. This study evaluated the fertility rate obtained in Merino ewes inseminated with ram semen stored at 5 °C in a TRIS-based extender in Uruguayan field conditions. The effect of storage period of semen (Experiment 1) and d...