A simple capillary enzymatic biosensor was developed. This was prepared by simply coating a dissolvable membrane containing enzyme/s on the inner wall of a square glass capillary. An easy measurement was carried out by capillary force sample introduction with concurrent enzyme release and a reaction with a certain substrate. Enzyme-release capillary (ERC) biosensors showed long-term storage sta...

The human bacterial pathogen Listeria monocytogenes is emerging as a model organism to study RNA-mediated regulation in pathogenic bacteria. A class of non-coding RNAs called CRISPRs (clustered regularly interspaced short palindromic repeats) has been described to confer bacterial resistance against invading bacteriophages and conjugative plasmids. CRISPR function relies on the activity of CRIS...

dans cet article i' intervalle entre deux trains successifs a ete etudie en fonction de leur vitesses dans deux cas differents. dans ie premier cas ,on suppose que la vitesse des deux trains a une valeur constante, et on etudie les variations de l'intervalle minimum en fonction de la vitesse on determine la vitesse optimale et on etudie les variations de l'intervalle minimum en f...

Carbonic anhydrase (CA) is an enzyme of high interest due to its implications relative the medical and environmental sectors. In current paper, assay for determination CA activity proposed it characterized by simplicity practicability. It permits straightforward comparison CAs performance in physiological conditions. The methodology theoretical background evaluation method are explained detail....

The N-terminal region of a 32 kDa cell-surface-binding protein, encoded by the D8L gene of vaccinia virus, shows sequence homology to CAs (carbonic anhydrases; EC 4.2.1.1). The active CAs catalyse the reversible hydration of CO2 to bicarbonate participating in many physiological processes. The CA-like domain of vaccinia protein [vaccCA (vaccinia virus CA-like protein)] contains one of the three...

Frozen sections of breast tumor tissue have been stained using an immunoperoxidase [estrogen receptor (ER)-immunocytochemistry] kit incorporating a monoclonal antiserum [H222] to visualize nuclear human ERs. Quantitation of specific staining has been performed by manual procedures using optical microscopy and by a computer-assisted image analysis system (CAS 100). Initial investigations with a ...

Abstract The widespread and versatile prokaryotic CRISPR–Cas systems (clustered regularly interspaced short palindromic repeats associated Cas proteins) constitute powerful weapons against foreign nucleic acids. Recently, the single-effector nuclease Cas12a that belongs to type V system was added enzymes repertoire employed for gene editing purposes. is a bilobal enzyme composed of REC Nuc lobe...

CRISPR-Cas is an adaptive prokaryotic immune system, providing protection against viruses and other mobile genetic elements. In type I and type III CRISPR-Cas systems, CRISPR RNA (crRNA) is generated by cleavage of a primary transcript by the Cas6 endonuclease and loaded into multisubunit surveillance/effector complexes, allowing homology-directed detection and cleavage of invading elements. Hi...