In the present study, modified indigenous ELISA kit (kit 1) was compared with indigenous ELISA kit (kit 2) and commercial ELISA kit (kit 3) for its sensitivity and specificity with respect to faecal culture for diagnosis of Johne's disease in goats and sheep under natural conditions. Of the 64 positive animals, serum of 42.1, 48.4 and 18.7% animals yielded positive infection in kit 1, 2 and 3, ...

BACKGROUND Haemophilus influenzae type b (Hib) infection has high morbidity and mortality rate, especially in children under 5 years of age. Enzyme-linked immunosorbent assay (ELISA) technique is the most used method to detect antibodies against H. influenzae. Available commercial ELISA kits are expensive and not always readily available, particularly for epidemiological studies. OBJECTIVES T...

A commercial M antibody capture ELISA kit ( Rubenz M) for the detection of rubella specific IgM was evaluated in comparison with M antibody capture radioimmunoassay. A total of 248 sera were evaluated, including sera from cases of primary postnatal rubella, congenital rubella, infectious mononucleosis, and sera which contained rheumatoid factor. No false positive results were obtained but two h...

Primary, secondary and even tertiary dengue infections can be detected taking into account the existence of four serotypes. During a primary infection, individuals develop IgM antibodies 5–6 days after the onset of illness lasting 30 to 90 days. IgG levels rise after IgM and are detectable for life[7]. During a secondary infection IgM levels are lower and in some cases absent. In contrast, IgG ...

BACKGROUND Currently, no dengue NS1 detection kit has regulatory approval for the diagnosis of acute dengue fever. Here we report the sensitivity and specificity of the InBios DEN Detect NS1 ELISA using a panel of well characterized human acute fever serum specimens. METHODOLOGY/PRINCIPAL FINDINGS The InBios DENV Detect NS1 ELISA was tested using a panel composed of 334 serum specimens collec...

The avidin-biotin enzyme-linked immunosorbent assay (A-B ELISA), for use in surveillance for bovine brucellosis in India was developed and calibrated using the indirect brucellosis ELISA kit of the International Atomic Energy Agency (IAEA) as a reference. The reagents used in the A-B ELISA were as follows: the smooth lipopolysaccharide of Brucella abortus strain 99 (antigen); biotinylated anti-...

Background: A number of studies have also reported that preexisting diabetes as well newly diagnosed with a first glucose measurement on hospital admission are both associated an increased risk all-cause mortality. This study aimed to investigate the immunological characteristics patients severe COVID-19 mellitus. Methods: was carried out in period between November 2021 and March 2022 Iraq. tot...

AIMS/INTRODUCTION The aims of the present study were to investigate the performance of a novel sandwich enzyme-linked immunosorbent assay (ELISA) for measuring glucagon (1-29) with monoclonal antibodies against both the C- and N-terminal regions of glucagon (1-29), and to analyze the differences in plasma levels and responses of glucagon (1-29) to oral glucose loading in normal glucose toleranc...

BACKGROUND Accurate and reliable diagnosis of HIV plays a central role in any effective HIV intervention. We decided to evaluate 4 commercial HIV test kits to determine their reliability for use in developing countries. METHODS Serum samples obtained from clients accessing tertiary health services at the STI clinic, Jos University Teaching Hospital were used to evaluate Sdbioline, Diaspot, De...

AND EXPLANATION OF THE TEST Detection of the Epstein-Barr virus was first described in 1964 by Epstein, Achong, and Barr using electron microscopic studies of cultured lymphoblasts derived from patients with Burkitt’s lymphoma. EBV is classified as a member of the herpes-virus family based upon it’s characteristic morphology. EBV infection may demonstrate a wide spectrum of clinical symptoms. T...