We have performed a physiological analysis of the effects of high-osmolarity media on gas1Delta cells. The reductions in the duplication time, number of pluribudded cells, hypersensitivity to Calcofluor and sodium dodecyl sulfate, and chitin level indicate a partial suppression of the mutant phenotype. GAS1 deletion was found to be lethal in the absence of the Bck1 and Slt2 (Mpk1) proteins of t...

This study reports the presence of filamentous fungi (ff) in drinking water including biofilms. Ff are not studied sufficiently in drinking water. Ff were highest in winter and had an indirect relation with other microorganisms. Pathogenic fungi were not observed at the mesophilic temperatures used. Penicillium expansum and P. brevicompactum were observed which may affect biofilms by mycotoxin ...

The cell wall secreted immediately following sperm entry into an egg can be visualized by the fluorescent dye Calcofluor white. Cell wall secretion precedes nuclear fusion by 10-20 min. SEM observations of the surface of unfertilized and fertilized eggs and sperm attachment to eggs are described. These results are discussed in relation to fertilization in sea urchins and the biochemical phenome...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 208 No. 6 729–744 www.jcb.org/cgi/doi/10.1083/jcb.201412024 JCB 729 Correspondence to Susan R. Wente: [email protected] Abbreviations used in this paper: ANOVA, analysis of variance; CCFW, calcofluor white; cNLS, classical NLS; DR, dietary restriction; ERC, extrachromosomal ribosomal DNA circle; HGPS, Hutchinson–Gilford progeri...

Of the various components of barley and oats, one constituent of the cereal cell wall, (1→3,1→4)-β-D-glucan (β-glucan), is important to a wide range of interests. Malting barley breeders strive to develop lines that modify well, yielding malts with low soluble β-glucan levels in Congress worts (less than the American Malting Barley Association guidelines of 100–120 ppm) (http:// www.ambainc.org...

Encystment is an essential process in the biological cycle of the human parasite Entamoeba histolytica. In the present study, we evaluated the participation of E. histolytica Gln6Pi in the formation of amoeba cyst-like structures by RNA interference assay. Amoeba trophozoites transfected with two Gln6Pi siRNAs reduced the expression of the enzyme in 85%, which was confirmed by western blot usin...