A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-l-gulonic acid (2-KLG). The screening methodology took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Using the proposed method, a mutant, K. vulgare 65, was selected from 20,000 colonies produced by a stra...

• Bromothymol blue's spectral properties were recorded in the presence of heavy water and some selected electrolytes. The removal dissolved gases from dye solution modifies its UV–vis absorption spectrum. Re-admitted induce specific effects on features dye. color pH indicator blue (BTB) changes yellow to with increasing pH. effect electrolytes (LiCl, NaCl, KCl, CsCl, KSCN KClO 3 ) D 2 O dilute ...