Photobleaching for CLEM experiments In order to effectively remove all FM1-43 derived photoconversion products in the bleach region of CLEM experiments, we scanned our rectangular region(s) of interest ten times at maximal laser power. In samples fixed immediately after photobleaching > 99% of vesicles in the bleach region of serial sections were PC− (Fig. 3a). We established that the bleach pr...

We investigated the spread of bleaching adaptation for human cone vision in the central fovea and at an eccentricity of 5 deg in the nasal retina. Cone thresholds measured after adaptation to a grating bleach were compared to those measured after a uniform bleach. We conclude that the foveal and parafoveal cone systems show excellent localization of the effects of adaptation. For areas 2.5-5 mi...

BACKGROUND Laos has a high prevalence of tuberculosis (TB) and a slowly increasing prevalence of human immunodeficiency virus/acquired immunedeficiency syndrome (HIV/AIDS). Sputum smear microscopy is the only method currently available for routine screening of pulmonary TB, although it only detects one in three cases among persons living with HIV (PLWH). Bleach treatment of sputum samples (blea...

BACKGROUND The emergence of Clostridium difficile as a major nosocomial pathogen points to the need for safe, effective, and fast-acting environmental sporicides for infection prevention and control. Available and fast-acting sporicides are generally corrosive and unsafe for both humans and the environment. METHODS We evaluated chlorine bleach (500 and 5000 ppm) and a gel containing 4.5% of a...

The biobleaching efficiency of xylanase and laccase enzymes was studied on kraft pulps from wood and nonwood based raw materials employed in the Indian paper industry. Treatment of these pulps with xylanase enzyme could result in improved properties, showing 2.0% ISO gain in pulp brightness and/or reducing the demand of chlorine-based bleach chemicals by up to 15% with simultaneous reduction of...

A common first step in time series signal analysis involves digitally filtering the data to remove linear correlations. The residual data is spectrally white (it is "bleached"), but in principle retains the nonlinear structure of the original time series. It is well known that simple linear autocorrelation can give rise to spurious results in algorithms for estimating nonlinear invariants, such...

of bleach and infections in children: a multicentre cross-sectional study' We write to express disappointment with the design flaws and speculative nature of the conclusions in the short report presenting findings from a study of domestic use of bleach and infections in children. 1 The article concludes that " Passive exposure to cleaning bleach in the home may have adverse effects on school-ag...

Children with probable community-associated Staphylococcus aureus skin and soft tissue or invasive infections were randomized to routine daily hygienic measures with or without "bleach baths" twice a week for 3 months. Within 12 months, a medically attended recurrence occurred in 84 of 495 (17%) children using bleach baths compared to 103 of 492 (21%) of control participants (P = .15).