1. A simple axenic medium suitable for the growth of the myxamoebae of a strain of the cellular slime mould Dictyostelium discoideum is described. 2. Procedures suitable for the growth of this strain in liquid and on solid media are described. 3. Conditions suitable for initiating the cell differentiation of myxamoebae grown axenically are described.

A single strain of Pseudo-nitzschia multiseries was grown axenically and in parallel cultures to which selected bacterial species isolated previously from P. multiseries cultures were added (non-axenic culture), an Alteromonas sp. (a producer of gluconic acid/gluconolactone when grown with glucose) and a Moraxella sp. (a non-producer of gluconic acid/gluconolactone). When gluconic acid/gluconol...

BACKGROUND The main goal of the present study was to set up an axenic cultivation of Acanthamoeba and assess the pathogenic ability of T4 genotypes from different clinical and environmental strains of Acanthamoeba using two physical assays. METHODS Sixteen Acanthamoeba isolates including 10 environmental and 6 clinical strains were cultured axenically. Axenic cultivation was performed using P...

The mushroom (Agaricus bisporus) has a requirement for a "casing layer" that has specific physical, chemical and microbiological properties which stimulate and promote the initiation of primordia. Some of these primordia then may develop further into sporophores, involving differentiation of tissue. Wild and commercial strains of A. bisporus were cultured in axenic and nonaxenic microcosms, usi...

1. The specific activities of fl-N-acetylglucosaminidase, acid phosphatase, ac-mannosidase, P-glucosidase, UDP-glucose pyrophosphorylase and alkaline phosphatase have been determined in myxamoebae of the cellular slime mould Dictyostelium discoideum Ax-2 grown on different media and in different phases of the growth cycle. 2. Variations in enzymic composition occur with changes in growth medium...

Although many isolation techniques for algaeaxenic cultures of algae are known, successful approaches species and contaminant-specific. The commercially important alga Haematococcus lacustris has been intensively studied due to its natural production the high-value carotenoid astaxanthin, yet there no reports axenic H. cultures. In this article, we describe lacustris, originally isolated in non...

In this study we examined whether the maintenance of Trypanosoma cruzi by long-time in axenic culture produces changes in gene expression and antigenic profiles. The studies were made with a Dm30L-clone from a low-virulent strain and a non-cloned virulent EP-strain of T. cruzi. Both parasites were maintained, for at least seven years, by successive alternate passage triatomine/mouse (triatomine...

Giardia lamblia cysts were isolated from fecal samples of 55 symptomatic and asymptomatic patients with giardiasis. The cysts were harvested and purified using the sucrose gradient method. IS The excystation of G. lamblia was obtained by treating them with HCI (0.01N)', then they were transferred on to modified TYI-S-33 medium. Out of 55 stool samples 17 cases were excysted with a rate of ...