herpes simplex virus 1 (hsv-1) unspliced 8.3 latency associated transcript (lat), which located in the long repeat sequences, has been shown to contain at least 16 open reading frames (orf: a-p). one of these orf, orf p, maps almost entirely antisense to hsv-1 neurovirulence gene, icp34.5. both orf p and icp34.5 are located in the long repeat and are antisense overlapping genes. therefore, in o...

Roses are among the most widely produced cut flowers, with important ornamental and economic value in flower market. In order to investigate how regulate flowering periods, negative regulator of GA biosynthesis RcSPY was cloned from Rosa chinensis ectopically expressed tobacco. The overexpression SPY4 transgenic tobacco significantly increased plant height. Antisense interference conserved SPY ...

Antisense oligonucleotides (ASOs) are a promising clinical tool that could be applied for unmet medical needs, but there several limitations their therapeutic application. Here, we designed and synthesized ( S )-5?- C -aminopropyl-2?- O -methylcytidine, containing -methyluridine -methylcytidine. We then investigated the properties of ASOs these nucleoside analogs. -Aminopropyl modifications enh...

To investigate the effect of long non coding RNA MAGI2-antisense 3 on proliferation, invasion and migration cervical cancer cells underlying mechanism. SiHa were divided into plasmid cloning DNA group, DNA-MAGI2-antisense anti-microRNA-negative control anti-microRNA-31-5p 3+microRNAnegative group 3+microRNA-31-5p group; quantitative reverse transcription polymerase chain reaction was used to de...

Gene therapy is in its development stage as a novel method for cancer treatment. Liposomes look promising as gene delivery vectors; however, investigations have shown that these vesicles are not doing well in some cases. It was decided here to investigate the possibility of augmentation of liposomal gene delivery by chemical penetration enhancers.Cationic liposome containing antisense oligonucl...

Modified 8–17 DNAzymes that can induce target gene silencing in cells were developed based on the X-ray crystal structure of DNAzyme and modifications used designing antisense oligonucleotides.

It was first predicted in 1988 that there may be an Open Reading Frame (ORF) on the negative strand of Human Immunodeficiency Virus type 1 (HIV-1) genome could encode a protein named AntiSense Protein (ASP). In spite some controversy, reports began to emerge years later describing detection HIV-1 antisense transcripts, presence ASP transfected and infected cells, existence immune response targe...

Antisense transcription is a prevalent feature at mammalian promoters. Previous studies have primarily focused on antisense transcription initiating upstream of genes. Here, we characterize promoter-proximal antisense transcription downstream of gene transcription starts sites in human breast cancer cells, investigating the genomic context of downstream antisense transcription. We find extensiv...

BACKGROUND Although genome-wide transcriptional analysis has been used for many years to study bacterial gene expression, many aspects of the bacterial transcriptome remain undefined. One example is antisense transcription, which has been observed in a number of bacteria, though the function of antisense transcripts, and their distribution across the bacterial genome, is still unclear. METHOD...