نتایج جستجو برای: alkaline comet assay

تعداد نتایج: 265321  

Journal: :Aquatic toxicology 2015
C Pérez-García J Rouxel F Akcha

In this work, the DNA-damaging effect of hydrogen peroxide on the structural integrity of nucleolar organizer regions (NORs) was studied for the first time by comet-FISH in the Pacific oyster Crassostrea gigas. Global DNA damage was assessed in hemocytes using an alkaline version of the comet assay. Next, NOR sensitivity was analyzed by mapping major rDNA repeat unit by fluorescence in situ hyb...

Journal: :Acta biochimica et biophysica Sinica 2013
Yali She Yi Li Yongqi Liu Guli Asai Shaobo Sun Jianxin He Zheng Pan Yan Cui

In this study, we assessed the toxic effects of formaldehyde (FA) on mouse bone marrow mesenchymal stem cells (BM-MSCs). Cytotoxicity was measured by using MTT assay. DNA strand breakage was detected by standard alkaline comet assay and comet assay modified with proteinase K (PK). DNA-protein crosslinks (DPCs) were detected by KCl-SDS precipitation assay. We found that FA at a concentration fro...

2002
M. Trevor Page Penelope J.E. Quintana Joseph A. Ligutti Roger A. Sabbadini

Apoptosis is a form of programmed cell death that has only recently been implicated in the pathogenesis of cardiac diseases, including ischemia and reperfusion damage and in response to cytokines and other apoptotic triggers. Methods to study apoptosis range from measures of early changes in morphology to late events such as DNA laddering. In studying apoptosis in the heart, the application of ...

Journal: :Mutagenesis 1996
P Fortini G Raspaglio M Falchi E Dogliotti

The single cell gel electrophoresis (SCGE) or comet assay, which measures DNA strand breaks in individual cells, was used to analyse DNA damage and repair induced by the SN1-type alkylating carcinogens N-ethyl-N'-nitro-N-nitrosoguanidine and N-ethyl-N-nitrosourea in CHO cells. The comet assay was comparable in sensitivity to the alkaline elution assay. The alkyl-adducts detected as DNA single-s...

Journal: :Mutagenesis 2010
Lykke Forchhammer Clara Johansson Steffen Loft Lennart Möller Roger W L Godschalk Sabine A S Langie George D D Jones Rachel W L Kwok Andrew R Collins Amaya Azqueta David H Phillips Osman Sozeri Maciej Stepnik Jadwiga Palus Ulla Vogel Håkan Wallin Michael N Routledge Catherine Handforth Alessandra Allione Giuseppe Matullo João Paulo Teixeira Solange Costa Patrizia Riso Marisa Porrini Peter Møller

The comet assay has become a popular method for the assessment of DNA damage in biomonitoring studies and genetic toxicology. However, few studies have addressed the issue of the noted inter-laboratory variability of DNA damage measured by the comet assay. In this study, 12 laboratories analysed the level of DNA damage in monocyte-derived THP-1 cells by either visual classification or computer-...

Journal: :jundishapur journal of natural pharmaceutical products 0
heibatullah kalantari school of pharmacy, toxicology research center, jundishapur university of medical sciences, ir iran +98-6113362197, [email protected]; school of pharmacy, toxicology research center, jundishapur university of medical sciences, ir iran +98-6113362197, [email protected] hamid galehdari school of pharmacy, toxicology research center, jundishapur university of medical sciences, ir iran +98-6113362197, [email protected] zahra zaree school of pharmacy, toxicology research center, jundishapur university of medical sciences, ir iran +98-6113362197, [email protected] hadi kalantar school of pharmacy, toxicology research center, jundishapur university of medical sciences, ir iran +98-6113362197, [email protected] golnaz varnasary school of pharmacy, toxicology research center, jundishapur university of medical sciences, ir iran +98-6113362197, [email protected]

background the increasing use of herbal drugs and their easy availability have necessitated the use of mutagenicity tests to analyze their toxicity and safety. objectives the aim of this study was to determine the in vitro mutagenicity of artemisia draconculus l., a herbal drug, by performing single cell gel electrophoresis (scge). materials and methods in this study, we obtained a herbal drug ...

2013
Xiang-Rong Xu Jun-Quan Zhu Ting Ye Chun-Lin Wang Yi-Feng Zhu Hans-Uwe Dahms Fan Jin Wan-Xi Yang

Although the single-cell gel electrophoresis (SCGE) alkaline comet assay can detect DNA damage quickly and with high sensitivity, it does not work well for cryopreserved sperm of some marine teleosts. Using large yellow croaker Pseudosciaena crocea sperm that were cryopreserved in increasing concentrations (5 to 30%) of DMSO, we made modifications to the classic SCGE method that included using ...

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