Liver enzyme levels are commonly obtained in the evaluation of many conditions. Elevated alanine transaminase and aspartate transaminase have traditionally been considered a "hepatocellular" pattern concerning for ischemic, viral, or toxic hepatitis. Elevations in these levels pose a diagnostic dilemma in patients without a clinical picture consistent with liver disease. On the other hand, elev...

Recently assays of glycolytic enzymes and transaminases have significantly expanded the diagnostic possibilities of internal medicine. Thus one may confirm myocardial infarction by serum transaminase activity within the first few hours in at least 95 per cent. of all cases. The testing of these enzymes has also improved the diagnosis of virus hepatitis and other liver diseases, and their undoub...

We determined transaminases in human blood serum with an amperometric glutamate biosensor. The probe was a hydrogen peroxide sensor assembled with appropriate selective membranes to enhance the probe specificity and lifetime. Calibration curves of glutamate were linear in the range 1-1000 mumol/L, with a response time of < 1 min. This probe was subsequently applied to the measurement of activit...

The transepithelial electrical potential difference across the isolated midgut of Bombyx mori larvae is dependent on the presence of potassium and is unaffected by the addition of hexoses to perfusion media, whereas it is enhanced by alanine, aspartic acid, glutamic acid and the corresponding 2oxoacids, glutamine and malate. The midgut enzyme profile indicates that the substrates for the tricar...

Tracer quantities (in 0.2 ml) of 13N-labeled glutamate, alanine, or glutamine(amide) were administered rapidly (less than or equal to 2 s) via the portal vein of anesthetized adult male rats. Liver content of tracer at 5 s was 57 +/- 6 (n = 6), 24 +/- 1 (n = 3), and 69 +/- 7 (n = 3)% of the injected dose, respectively. Portal-hepatic vein differences for the corresponding amino acids were 17 +/...

the anti-apoptotic gene bcl-2 is located in mitochondria, but it is uncertain whether its expression affects hepatocyte survival in ischemia/reperfusion (i/r) injury. this experiment was designed to evaluate the role of folic acid in expression of bcl-2 in i/r in rat liver. eighteen wister rats were divided into sham-operated controlgroup (c) (n=6), i/r group (n=6), folic acid treated group whi...

The article is devoted to the study of assessment selenium status in pathogenesis liver diseases and effectiveness use a selenium-containing drug. In course studies animals with clinical signs high rates hepatopathies markers – transaminases, 100% cases low level was revealed 0.026 ±0.01 μg / ml, because increased consumption this microelement on background free radical oxidation processes tiss...

In a study of the composition of the epididymal fluid of rams, attention was given to the fact that nitrogen compounds constitute a great part of the dry matter and that so far seventeen free amino acids have been detected in the fluid (Ch. Neumark, unpublished data). It was therefore deemed of interest to study the enzymes of their metabolism. Two principal transaminases were investigated: glu...