c-Jun is considered a major regulator of both neuronal death and regeneration. Stress in primary cultured CNS neurons induces phosphorylation of c-Jun serines 63 and 73 and increased c-Jun protein. However, total c-Jun N-terminal protein kinase (JNK) activity does not increase, and no satisfactory explanation for this paradox has been available. Here we demonstrate that neuronal stress induces ...

AP-1, the polypeptide product of c-jun, recognizes and binds to specific DNA sequences and stimulates transcription of genes responsive to certain growth factors and phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA). We studied the effects of TPA on the regulation of c-jun gene expression in HL-60 cells during monocytic differentiation. Low levels of c-jun transcripts were detec...

Upregulation of miR-744 is associated with poor prognosis in many types of cancer patients, but it is still unclear how miR-744 becomes elevated in these tumors. In this study, we found that ectopic c-Jun elevated miR-744 expression, whereas c-Jun attenuation reduced miR-744 expression. Chromatin immunoprecipitation assay confirmed the direct binding of c-Jun to the promoter of miR-744. The bin...

Ventricular myocytes are exposed to various pathologically important cell stresses in vivo. In vitro, extreme stresses (sorbitol-induced hyperosmotic shock in the presence or absence of okadaic acid, and anisomycin) were applied to ventricular myocytes cultured from neonatal rat hearts to induce a robust activation of the 46 and 54 kDa stress-activated protein kinases (SAPKs). These activities ...

We have previously identified (M. Wang et aL, OncoL Res., in press, 1998) an enhancer element [human tissue inhibitor of metalloproteinase-1 enhancer-i (HTE)] for the human tissue inhibitor of metalloproteinase-1 promoter that binds a novel zinc finger, cysteine-rich transcription factor (CRTF). In this study, we have used electrophoretic mobifity shift assays to examine the relative level of e...

The cyclin-dependent kinase (cdk) inhibitor p27 is a central mediator in the imposition and maintenance of quiescence through the sequestration of G1-specific cyclin-cdk complexes. Previous studies have implicated the c-Jun co-activator protein Jab1 as a regulator of intracellular p27 levels. Jab1 has been reported to interact with p27 and cause its translocation to the cytoplasm as a prelude t...

Correction for ‘Spherical Co 3 S 4 grown directly on Ni–Fe sulfides as a porous nanoplate array FeNi foam: highly efficient and durable bifunctional catalyst overall water splitting’ by Jun Shen et al. , J. Mater. Chem. A 2022, 10 5442–5451, DOI: 10.1039/d1ta07504g.

background: the mitogen-activated protein kinase (mapk) pathway is one of the major signaling pathways that transmit intracellular signals initiated by extracellular stimuli to the nucleus. the stress-activated protein kinase (sapk)/c-jun nh2-terminal kinase is a subfamily of map kinases implicated in cytokine and stress responses. objective: in this study, we have examined total and phosphoryl...

IL-12p70 is an immunoregulatory cytokine that has been shown to induce IL-10 production from CD4+ T cells, yet the underlying cellular mechanisms controlling this process are poorly understood. In the present study, we demonstrate that IL-12p70 induces IL-10 production from human memory CD4+ T cells via a PI3K-dependent signaling mechanism. Specifically, stimulation of human memory CD4+ T cells...

Co-I Braun was on extended family leave for much of this quarter, due to the birth of his daughter on May 10. Other members spent considerable time preparing for, and attending, a number of summer workshops and conferences. These included the conference on “MHD Waves and oscillations in solar magnetic structures” in Palma de Mallorca, Spain (May 29 Jun 1), the SPD meeting in Durham, New Hampshi...