measles virus (mv) is a highly contagious agent which causes a major health problem in developing countries. we studied the effect of buthionine sulfoximine (bso) on the replication of an aik-hdc strain of mv and its induced apoptosis in vero cell lines. methods: in this study, toxicity of bso on vero cells was investigated first, resulted in determination of sub-lethal or non-toxic concentrati...

Outbreaks of Zika virus (ZIKV) have resulted in a call by global health advocates for increased surveillance and research with aggressive measures to combat ZIKV infections. There is no licensed vaccine yet available, but number candidates are development. Advancement promising licensure may rely upon the development use well-characterized preclinical models developed based on essential element...

Recognition of the infectivity of ribonucleic acid (RNA) of tobacco mosaic (1, 2) and of animal virus (3) initiated investigation of the molecular aspects of viral reproduction. Study of replication of infectious RNA depends upon employment of efficient and reproducible biological assay procedures. Reception of viral genetic material normally depends upon specific interaction of cellular and vi...

OBJECTIVES Enterovirus 71 (EV71) causes serious diseases in humans. The aim of this study was to examine the effects of aurintricarboxylic acid (ATA) on EV71 replication and to explore the underlying mechanism. METHODS To measure the activity of ATA in inhibiting the cytopathic effect (CPE) of EV71, a cell-based neutralization (inhibition of virus-induced CPE) assay was performed. The effect ...

A small molecule library containing 480 known bioactive compounds was screened for antiviral activity against poliovirus (PV) using a cellular fluorescence resonance energy transfer (FRET) assay for viral protease activity. The infected reporter cells treated with the viral replication-suppressing compounds were examined via fluorescence microscope 7.5 h postinfection. Twelve molecules showed m...

background: during the recent years, significant progress has been achieved on development of novel anti-viral drugs. natural products are assumed as the potential sources of novel anti-viral drugs; therefore, there are some previous studies reporting the anti-viral compounds from venomous animals. based on the significant value for tracing of non-toxic anti-viral agents from natural resources,...

Male-specific coliphages are often used as indicators of contamination by enteric viruses. These phages can be detected in water samples by plaque assays and by polymerase chain reaction. In this study, the M13 coliphage was used to develop a real-time PCR assay for the detection of male-specific DNA coliphages. The real-time PCR was found to have a reaction efficiency of 1.45 and detection lim...

This new assay of viral cell-mediated immunity is sensitive, reproducible, and in many ways resembles the in vivo state. The spread of herpes simplex virus between adjacent monolayer cells was inhibited in the presence of spleen cells from guinea pigs sensitized to that virus. This in vitro control of viral growth was quantified by determining plaque size in monolayers to which were added sensi...

Foci of cells infected with human cytomegalovirus were noted to stain more intensely than uninfected cells with neutral red, and this provided the basis for development of a plaque assay and plaque reduction neutralization test for cytomegalovirus. Plaques demonstrable by neutral red staining could be counted at 8 days after infection; thus, results could be obtained earlier than for plaque ass...

An in situ enzyme-linked immunosorbent assay (ELISA), performed directly on fixed varicella-zoster virus-infected monolayers, was used to quantitate viral antigen, and by color reduction it was used to evaluate the activity of drugs against varicella-zoster virus. Color production in the ELISA (optical density) was directly related to the dose of input virus. Antigen representing 5 to 10 plaque...