conclusions therefore the non-infectious dlps were became infectious if introduced into the cytoplasm of permissive and cancerous cells, without passing attachment and entry process. results we attempt to avoid the attachment and entry of the rotavirus by using lipofectamine to mediate the delivery of viral particles directly into the cytoplasm. dlp was endocytosed into the cytoplasm following ...

objective: titration of viruses is important to determine the quantity of virus in vaccine development, master virus seed stock preparation, viral vector studies and virus replication. in this study, we compared the ccid50% and plaque assay as a standard titration method for rotavirus (rf) and hsv-1.   methods: the ma104 and vero cells were inoculated by rf and hsv-1 in 6- and 96-well plates. f...

background and aims: inhibition of viral growth in coinfected cells with two different viruses has been described. this phenomenon known as viral interference can occur in several virus host systems such as interference of enterovirus infection on poliovirus vaccine strains. in this study we superinfected reovirus infected hela cells with poliovirus to determine if poliovirus can replicate in s...

Background and Aims: Inhibition of viral growth in coinfected cells with two different viruses has been described. This phenomenon known as viral interference can occur in several virus host systems such as interference of enterovirus infection on poliovirus vaccine strains. In this study we superinfected reovirus infected HeLa cells with poliovirus to determine if poliovirus can replicate in s...

Murine norovirus (MNV) is the only member of the Norovirus genus that efficiently grows in tissue culture. Cell lysis and cytopathic effect (CPE) are observed during MNV-1 infection of murine dendritic cells or macrophages. This property of MNV-1 can be used to quantify the number of infectious particles in a given sample by performing a plaque assay. The plaque assay relies on the ability of M...

Lymphocytic choriomeningitis virus (LCMV), a natural murine pathogen, is a member of the Arenavirus family, may cause atypical meningitis in humans, and has been utilized extensively as a model pathogen for the study of virus-induced disease and immune responses. Historically, viral titers have been quantified by a standard plaque assay, but for non-cytopathic viruses including LCMV this requir...

Two newly developed protocols for infective virus detection were compared to the plaque assay. An immunomagnetic separation procedure coupled with real-time reverse transcription-PCR of viral nucleic acids was developed to identify intact enteroviral particles, and a reporter cell system responding to viral replication based on fluorescent resonance energy transfer for detection of infectious e...

An overnight assay, based on staining cytomegalovirus-infected cells with monoclonal antibody to the 72,000-molecular-weight major immediate-early viral protein, was compared with a conventional 14-day plaque assay for quantitation of cell-free stocks of cytomegalovirus laboratory strain AD-169 and 20 other clinical strains. Viral titers were quantitatively similar when determined by either met...

tion method reported by Sussman (5) and practically sufficient to determine the inoculum size of baculovirus for plaque isolation or large-scale protein expression. Based on these observations, we replaced the plaque formation assay with the β-gal activity assay to obtain viral titers in our routine laboratory practice. We have successfully shortened the periods of time from transfection to pre...

The plaque assay is a common technique used to measure virus concentrations and is based upon the principle that each plaque represents a single infectious unit. As such, the number of plaques is expected to correlate linearly with the virus dilution plated, and each plaque should be formed by a single founder virus. Here, we examined whether more than one virus can contribute to plaque formati...