BENCHMARKS It has been nearly three decades since the invention of DNA electro-phoresis (1−6). Current conductive media for DNA electrophoresis remain largely restricted to legacy Tris-acetic acid-disodium EDTA (TAE) and Tris-boric acid-disodium EDTA (TBE) at substantial ionic strengths, leading to higher cost and excessive heat generation , and limiting the voltage and speed of electrophoretic...

addressed some recently recognized problems in testing concentrated urine for monoclonal free light chains of immunoglobulins, i.e., Bence Jones protein (BJP), by high-resolution elec-trophoresis (lIRE) and immunofix-ation electrophoresis (IFE). When BJP is present in low concentrations, poly-clonal free light chains can interfere with identification of the protein either by causing a dense bac...

Chlorella, Chlorophyll-Protein Complexes, Polyacrylamide-Gel Electrophoresis, Standard Curves for Characterization of Chlorophyll Proteins Chlorophyll-protein complexes from thylakoids of the normal type and two mutants of Chlorella fusca were separated using sodium dodecyl sulfate acrylamide gel electrophoresis (PAGE). The properties of the chlorophyll-protein complexes of the three strains of...

1. Introduction Optical manipulation of biological cells in microfluidic devices is a powerful technique for high-throughput cell-based assays. It offers several advantages compared with other techniques: it is non-invasive, contamination free , and needs no or minimum fabrication for the mi-crofluidic devices. The most well-known optical manipulation tool is optical tweezers [1, 2]. To achieve...

155 sive text that is technically and scientifically well prepared. It is easy to appreciate the careful attention to detail that went into the planning and execution of this work. The dedication of this first volume in the Protein Abnormalities series to the physiology of immunoglobulins should come as no surprise in the face of the continued surge of research in this area. These highly divers...

β-lactamase, altered porins, or both are usually responsible for cefoxitin resistance in Escherichia coli. We examined the relative importance of each. We studied 18 strains of clinical isolates with reduced cefoxitin susceptibility and 10 initially-susceptible strains passaged through cefoxitin-gradient plates. Of 18 wild-resistant strains, 9 had identical promoter mutations (including creatio...

Photosystem I preparations were obtained from wild type tobacco Nicotiana tabacum var. John William’s Broadleaf (JWB) and from the two chlorophyll-deficient mutants N tabacum Su/su and N. tabacum Su/su var. Aurea. The preparations were characterized with respect to the chlorophyll a/b ratio, their photosynthetic activity and their absorption spectroscopic properties. Peptides from these prepara...

human leukocytes cause activating mutations in the K-ras protooncogene. Nakajima T. K-ras gene point mutation in neo-genetic lesions of subpleural fibrotic lesions: either an early genetic event in lung cancer development or a non-specific genetic change during the inflammatory reparative process. Topographic analysis of K-ras mutations in histologically normal lung tissues and tumours of lung ...

Combined megaloblastic anemia with thalassemia is easily masked because of the loss of macrocytosis. We performed a retrospective study to compare the major parameters in 4 groups of subjects in order to show the characteristics of patients with megaloblastic anemia and thalassemia. Group A comprised 9 patients with megaloblastic anemia and tha-lassemia, group B comprised 10 patients with uncom...

Biocheniical alterations in blood due to pathologic chaiges in the legs are difficult to detect when the blood samples are taken from the cubital vein. In the reported investigations biochemical properties of this blood are compared with those of the venous blood flowing out from a leg affected by obliterative atheroselerosis. A THEROMATOUS processes developing iii the walls of the arteries as ...