1. The oxidation of butyrate, hexanoate and octanoate by rat-liver mitochondria suspended in a tris-potassium chloride medium in the presence of malate and serum albumin has been investigated. 2. The oxidation of butyrate to acetoacetate was markedly decreased by the addition of a system competitive for ATP (hexokinase-glucose). 3. Serum albumin or tricarboxylic acid-cycle intermediates prevent...

The quantitative significance of the tricarboxylic acid cycle in the oxidation of acetate by yeast has remained in doubt despite many investigations concerning this topic. All of the enzymes of the tricarboxylic acid cycle have been demonstrated in either commercial baker's yeast or in pure cultures of Saccharomyces cerevisiae. (Weiland and Sonderhoff, 1932; Lynen and Neciullah, 1939; Novelli a...

The metabolic conversion of dicarboxylic acids into succinate and other gluconeogenic intermediates in rat liver homogenates was investigated using [1,2,4-13C4]dodecanedioic acid as tracer. Isotope enrichments in 3-hydroxybutyrate, succinate, fumarate, and malate, as well as dicarboxylates (dodecanedioic, sebacic, suberic, and adipic acids) were measured with selected ion monitoring capillary c...

Previous work has indicated that much of the biochemical maturation of the mammalian liver takes place around the time of birth (l-6). In the present study of this critical period, we have investigated the mode of pyruvate utilization in the tricarboxylic acid cycle. Pyruvate enters the tricarboxylic acid cycle in two ways, as acetyl coenzyme A and as a dicarboxylic acid. In the following exper...

Cocrystallization of 2-amino-benzothia-zole with benzene-1,2,4-tricarboxylic acid in a mixed solvent affords the title ternary cocrystal, C(7)H(7)N(2)S(+)·C(9)H(5)O(6) (-)·H(2)O, in which one of the carboxyl groups of the benzene-tricarboxylic acid is deproton-ated and the heterocyclic N atom of the 2-amino-benzothia-zole is protonated. In the crystal, inter-molecular N-H⋯O and O-H⋯O hydrogen-b...

During the past few years scattered information has appeared which indicates that at least some bacteria probably possess a tricarboxylic acid cycle. Lockwood and Stodola found that Pseudomonas jluorescens formed considerable quantities of a-ketoglutaric acid when growing on a glucose medium under conditions of intense aeration (1). Ajl and Werkman found evidence for at least parts of the conve...

During the past few years scattered information has appeared which indicates that at least some bacteria probably possess a tricarboxylic acid cycle. Lockwood and Stodola found that Pseudomonas jluorescens formed considerable quantities of a-ketoglutaric acid when growing on a glucose medium under conditions of intense aeration (1). Ajl and Werkman found evidence for at least parts of the conve...