BACKGROUND After the recent cloning of light-sensitive ion channels and their expression in mammalian cells, a new field, optogenetics, emerged in neuroscience, allowing for precise perturbations of neural circuits by light. However, functionality of optogenetic tools has not been fully explored outside neuroscience, and a nonviral, nonembryogenesis-based strategy for optogenetics has not been ...

OBJECTIVE To externally validate the prognostic models for predicting the time-dependent outcome in patients with locally advanced cervical cancer (LACC) who were treated with concurrent chemoradiotherapy in an independent cohort. METHODS A historical cohort of 297 women with LACC who were treated with radical concurrent chemoradiotherapy from 1999 to 2014 at the 12 de Octubre University Hosp...

Con el fin de determinar sentido responsabilidad social adquirido por estudiantes universitarios al finalizar Trabajo Comunal Universitario (TCU), se realizó una investigación mixta en la que describen y cuantifican frecuencias respuesta dadas estudiantado. Participaron 26 estudiantes, cada persona 300 horas intervención comunidad vez terminado su aporte completaron un cuestionario. Como princi...

Abstract Created in 1890, the Tribunal de Contas da União (TCU) is today one of most relevant institutions Brazilian State. This article explains how TCU became this very particular institution, from its historical origins as a simple court accounts to efforts differentiate itself model and gain other (many) powers, reshaping itself. In end, we present synthesis current debates on institutional...

Cell-to-cell communication was characterized in cumulus-oocyte complexes from rat ovarian follicles before and after ovulation. Numerous, small gap junctional contacts were present between cumulus cells and oocytes before ovulation. The gap junction are formed on the oocyte surface by cumulus cell processes that transverse the zona pellucida and contact the oolemma. The entire cumulus mass was ...

Precise control of gene expression is a powerful method to elucidate biological function, and protein overexpression is an important tool for industry and biochemistry. Expression of the Neurospora crassa tcu-1 gene (NCU00830), encoding a high-affinity copper transporter, is tightly controlled by copper availability. Excess copper represses, and copper depletion, via the use of a copper chelato...

Porcine immature oocyte quality (i.e., that of live oocytes at the germinal vesicle stage) was evaluated according to features of the surrounding cumulus, aiming to establish maturational competence of different subpopulations of such cumulus-oocyte complexes. Six subpopulations were identified: A1 (with a dense cumulus), A2 (with a translucent cumulus), B1 (with the corona radiata), B2 (partly...

OBJECTIVE To investigate the role of the cumulus cells and the cumulus matrix in affecting the penetrability, morphology, acrosome reaction, and motility of human spermatozoa penetrating the cumulus oophorus. DESIGN Controlled experimental laboratory study. SETTING University gynecology unit. PATIENT(S) Women undergoing assisted reproduction treatment and men visiting the subfertility cli...