The engineering of thermostable enzymes is receiving increased attention. The paper, detergent, and biofuel industries, in particular, seek to use environmentally friendly enzymes instead of toxic chlorine chemicals. Enzymes typically function at temperatures below 60°C and denature if exposed to higher temperatures. In contrast, a small portion of enzymes can withstand higher temperatures as a...

Geobacillus stearothermophilus SR74 is a locally isolated thermophilic bacteria producing thermostable and thermoactive α-amylase. Increased production and commercialization of thermostable α-amylase strongly warrant the need of a suitable expression system. In this study, the gene encoding the thermostable α-amylase in G. stearothermophilus SR74 was amplified, sequenced, and subcloned into P. ...

snail is one of the worst causes of food allergy. thus, the aim of this study was to identify the major and minor allergens of the local marine snail ( cerithidea obtusa ) and subsequently to investigate the impacts of heat treatment on the ige-binding activity of snail allergens. proteins from raw and heat-treated snails (boiled, roasted and fried) were extracted and then resolved by sodium do...

polyphenol oxidase (ec. 1.10.3.1 ppo), an ionically unbound and thermostable enzyme, was extracted from the leaves of cinnamomum tamala having aroma flavor used to add in indian food and vegetables as a part of spices to enhance the taste of recipes. the enzyme was purified 2.63-fold with a total yield of 9.5% by ammonium sulfate precipitation followed by sephadex g-100 gel filtration chromatog...

A vibrio isolated from the intestine of a coastal fish was identified as Vibrio hollisae by its biochemical characteristics. The isolate reacted with the gene probe for the thermostable direct hemolysin of Vibrio parahaemolyticus. The hemolysin produced by the isolate from the fish had traits identical to those of the thermostable direct hemolysin-like hemolysin produced by a clinical strain of...

By using a genetically modified thermostable protein (KTQ5C), we have synthesized protein-stabilized goldnanoclusters (AuNC@KTQ5C) with advantageous properties, such as heat stable fluorescent emission and resistant peroxidase-like activity.

Thermostable enzymes have applications in biosensors and bioreactors. Overexpression of thermostable enzyme-encoding genes from thermophiles Escherichia coli is necessary to produce sufficient quantities such enzymes. Deinococcus gerthermalis a moderately thermophilic bacterium has Nicotinamide adenine dinucleotide (NADH) an important coenzyme for various dehydrogenases. NADH oxidase (Nox) cata...

The adenosine A2A receptor (A2AR) belongs to the superfamily of membrane proteins called the G-protein-coupled receptors (GPCRs) that form one of the largest superfamilies of drug targets. Deriving thermostable mutants has been one of the strategies used for crystallization of A2AR in both the agonist and antagonist bound conformational states. The crystal structures do not reveal differences i...

BACKGROUND The concerted action of three complementary cellulases from Clostridium thermocellum, engineered to be stable at elevated temperatures, was examined on a cellulosic substrate and compared to that of the wild-type enzymes. Exoglucanase Cel48S and endoglucanase Cel8A, both key elements of the natural cellulosome from this bacterium, were engineered previously for increased thermostabil...