Three Pestalotiopsis isolates were obtained from leaves of Coffea arabica and Rhodomyrtus tomentosa. Among them, two isolates produced versicolorous conidia, and the other produced concolorous conidia. Phylogenetic analysis based on a combination of ITS, β-tubulin and tef1 gene sequence data clearly confirms that they belong to two species and distinguishes them from other species in this genus...

Early blight (EB) is a destructive disease affecting potato and tomato plants in Russia, caused by heterogeneous group of plant pathogenic Alternaria fungi. The current species delimitation sect. Porri with medium to large conidia long (filamentous) beak based on molecular data. In this study, the ITS, GAPDH, RPB2, TEF1, Alt 1 gene regions were analyzed 41 large-spored isolates obtained from di...

Bidirectional tRNA movement between the nucleus and the cytoplasm serves multiple biological functions. To gain a biochemical understanding of the mechanisms for tRNA subcellular dynamics, we developed in vivo β-importin complex coimmunoprecipitation (co-IP) assays using budding yeast. Our studies provide the first in vivo biochemical evidence that two β-importin family members, Los1 (exportin-...

The diversity of Trichoderma spp. was investigated in alkaline soils of pistachio orchards at different geographic areas in Kerman province, Iran. A total of 161 Trichoderma isolates were obtained and identified at the species level by their morphological characters and sequence analysis of their internal transcribed spacer (ITS) and tef1-α genomic regions. Totally, five species of Trichoderma ...

Molecular characterization of locally isolated Beauveria bassiana (Cordycipitaceae, Hypocreales) strains and their pathogenicity against Ceratitis capitata (Diptera: Tephritidae) adults are herein reported for the first time. Molecular identification based on the phylogenetic analyses of ITS-rDNA and combined data of TEF1 and Bloc, revealed that all fungal strains were conclusively assigned to ...

We developed a novel technique, called pseudouridine site identification sequencing (PSI-seq), for the transcriptome-wide mapping of pseudouridylation sites with single-base resolution from cellular RNAs based on the induced termination of reverse transcription specifically at pseudouridines following CMCT treatment. PSI-seq analysis of RNA samples from S. cerevisiae correctly detected all of t...

BACKGROUND Species identification is essential for controlling disease, understanding epidemiology, and to guide the implementation of phytosanitary measures against fungi from the genus Diaporthe. Accurate Diaporthe species separation requires using multi-loci phylogenies. However, defining the optimal set of loci that can be used for species identification is still an open problem. METHODS ...

Trichoderma viridescens is recognised as a species complex. Multigene analyses based on the translation elongation factor 1-alpha encoding gene (tef1), a part of the rpb2 gene, encoding the second largest RNA polymerase subunit and the larger subunit of ATP citrate lyase (acl1) reveals 13 phylogenetic species with little or no phenotypic differentiation. This is the first use of acl1 in Trichod...

Taxonomic studies of bambusicolous fungi in China and Thailand have resulted the collection three fascinating saprobic coelomycetes strains. Morphology coupled with combined gene analysis ITS, LSU, TUB2, TEF1-? DNA sequence data showed that they belong to genus Apiospora, family Apiosporaceae. A new species from Thailand, Apiospora mukdahanensis, records A. locuta-pollinis are herein described....