نتایج جستجو برای: tcid50
تعداد نتایج: 474 فیلتر نتایج به سال:
Background and purpose: Since long time ago herbal products and spices have been used as powders, essential oils (EOs), and extracts. They consist of antimicrobial components and could be applied as natural food preservatives. In the present work, the antiviral effect of cinnamon, rosemary, and zataria EOs has been investigated against norovirus surrogates including feline calicivirus (FCV) and...
Objective: To 1) characterize the decay curve of infective SARS-CoV-2 over time on surface cardboard packaging and plastic mailer packaging; 2) transferability virus-inoculated to skin. Methods: We inoculated samples with a titer > 106 TCID50/ mL evaluate survivability skin (pig skin) time. A cell culture-based infectivity assay (TCID50) was used determine viral titers. Regression analysis curv...
Human metapneumovirus (hMPV) is a newly described member of the Paramyxoviridae family causing acute respiratory tract infections, especially in young children. We studied the pathogenesis of this viral infection in two experimental small animal models (BALB/c mice and cotton rats). Significant viral replication in the lungs of both animals was found following an intranasal challenge of 10(8) 5...
BACKGROUND In 2008, an outbreak of canine distemper virus (CDV) infection in monkeys was reported in China. We isolated CDV strain (subsequently named Monkey-BJ01-DV) from lung tissue obtained from a rhesus monkey that died in this outbreak. We evaluated the ability of this virus on Vero cells expressing SLAM receptors from dog, monkey and human origin, and analyzed the H gene of Monkey-BJ01-DV...
This study reports the in vitro inhibitory potential of crude extract of Quercus lusitanica (Q. lusitanica) seeds on the replication of dengue virus type 2 (DEN-2). In vitro antiviral activity of Q. lusitanica extract, assessed in C6/36 cells (cloned cells of Aedes albopictus larvae) employing a virus inhibition assay, showed dose-dependent inhibition. The Q. lusitanica extract at its maximum n...
BACKGROUND Porcine epidemic diarrhea virus (PEDV), a highly pathogenic and transmissible virus in swine, was first detected in the U.S. in May, 2013, and has caused tremendous losses to the swine industry. Due to the difficulty in isolating and growing this virus in cell culture, few vaccine studies using cell culture propagated PEDV have been performed on U.S. strains in pigs. Therefore, the o...
The aim of the paper was to compare the real-time PCR with the heminested RT-PCR method, both applied for the detection of nucleoprotein gene of rabies viruses in bats and terrestrial animals. The study involved 32 rabies virus isolates collected from bats and terrestrial animals coming from different regions of Poland. For both methods, the sensitivities related to TCID50/mL of CVS virus were ...
In an attempt to evaluate the level of attenuation of live parainfluenza type 3 virus (PIV3) vaccine candidates, we compared the responses of partially immune adult volunteers inoculated intranasally with 10(6) to 10(7) 50% tissue culture infective dose (TCID50) of bovine PIV3 (n = 18) or cold-adapted (ca) PIV3 (n = 37) with those of 28 adults administered 10(6) to 10(7) TCID50 of wild-type PIV...
سابقه و هدف: فرآورده های گیاهی و ادویه جات، که از گذشته به صورت پودر، اسانس و عصاره مورد استفاده قرار گرفته اند واجد ترکیبات ضدمیکربی و قابلیت کاربرد به عنوان نگهدارنده های طبیعی خوراکی می باشند. در مطالعه حاضر، تاثیر ضدویروسی اسانس های دارچین، رزماری و آویشن شیرازی بر عفونت زایی سویه های نورو ویروس، کلسی ویروس گربه (fcv) و نوروویروس موش (mnv)، مورد بررسی قرار گرفت. مواد و روش ها: در این مطالعه...
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