Hearing someone refer to themselves in the third person may seem "wacky", but you may be surprised to learn that it can actually be pretty helpful to the person doing it. Some people find that speaking in third person improves their self-esteem, their ability to perform well under stress, to manage their emotions more favorably, and to think through complex situations in a more rational and cal...

Recent effective use of TAL Effectors (TALEs) has provided an important approach to the design and synthesis of sequence-specific DNA-binding proteins. However, it is still a challenging task to design and manufacture effective TALE modulators because of the limited knowledge of TALE-DNA interactions. Here we synthesized more than 200 TALE modulators and identified two determining factors of tr...

Recent work has shown that the combinatorial use of multiple TALE activators can selectively activate certain cellular genes in inaccessible chromatin regions. In this study, we aimed to interrogate the activation potential of TALEs upon transcriptionally silenced immune genes in the context of non-immune cells. We designed a unique strategy, in which a single TALE fused to the TATA-box binding...

Transcription activator-like effector (TALE) nuclease (TALEN) is widely used as a tool in genome editing. The DNA binding part of TALEN consists of a tandem array of TAL-repeats that form a right-handed superhelix. Each TAL-repeat recognises a specific base by the repeat variable diresidue (RVD) at positions 12 and 13. TALEN comprising the TAL-repeats with periodic mutations to residues at posi...

*Plant pathogenic bacteria of the genus Xanthomonas inject transcription activator-like effector (TALe) proteins that bind to and activate host promoters, thereby promoting disease or inducing plant defense. TALes bind to corresponding UPT (up-regulated by TALe) promoter boxes via tandemly arranged 34/35-amino acid repeats. Recent studies uncovered the TALe code in which two amino acid residues...

Bill Willingham’s Fables comic book series and its spin-offs have spanned fourteen years and reinforce that fairy-tale characters are culturally meaningful, adaptable, subversive, and pervasive. Willingham uses fairy-tale pastiche and syncreticism based on the ethos of comic book crossovers in his redeployment of previous approaches to fairy-tale characters. Fables characters are richer for eve...

Engineered TAL-effector nucleases (TALENs) and TALE-based constructs have become powerful tools for eukaryotic genome editing. Although many methods have been reported, it remains a challenge for the assembly of designer-based TALE repeats in a fast, precise and cost-effective manner. We present an ULtiMATE (USER-based Ligation Mediated Assembly of TAL Effector) system for speedy and accurate a...

Hearing someone refer to themselves in the third person may seem "wacky", but you may be surprised to learn that it can actually be pretty helpful to the person doing it. Some people find that speaking in third person improves their self-esteem, their ability to perform well under stress, to manage their emotions more favorably, and to think through complex situations in a more rational and cal...

Hearing someone refer to themselves in the third person may seem "wacky", but you may be surprised to learn that it can actually be pretty helpful to the person doing it. Some people find that speaking in third person improves their self-esteem, their ability to perform well under stress, to manage their emotions more favorably, and to think through complex situations in a more rational and cal...

Introduction ............................................................................... 357 I. A Brief History of Law and Development ................................ 361 A. Law and Development After 9/11: Development as National Security............................................................. 361 B. Development as Human Rights ....................................... 365 II. Culture a...