The gene encoding alpha fetoprotein (locus symbol Afp) was assigned to rat chromosome 14 at band p21-p22 using fluorescence in situ hybridization method. The present result suggests that there is a conserved syntenic group between human 4q11-q13, mouse 5F-G, and rat 14p21-p22.

Phages are promising alternatives to antibodies as the biorecognition element in a variety of biosensing applications. In this study, a monolayer of bacteriophage P22 whose tailspike proteins specifically recognize Salmonella serotypes was covalently bound to glass substrates through a bifunctional cross linker 3-aminopropyltrimethoxysilane. The specific binding of Salmonella typhimurium to the...

Previously, we have shown that p22, an EF-hand Ca2+-binding protein, interacts indirectly with microtubules in an N-myristoylation-dependent and Ca2+-independent manner. In the present study, we report that N-myristoylated p22 interacts with several microtubule-associated proteins within the 30-100 kDa range using overlay blots of microtubule pellets containing cytosolic proteins. One of those ...

‘l’l~e SWYW EcoKI restriction endonuclease cleavage sites in bacteriophage P%d DNA have been mapped. The cleavage site map of circularly permut.ed P22 linear DNA is a circle. The positions of EcoRI sites in the early region of the P22 genomc were determined by comparing products of EcoRI digestion of maturtx liaear P22 chromosomes with the EcoRI cleavage fragments of DNA of three XimmP22 hybrid...

The Salmonella enterica opvAB operon is a horizontally-acquired locus that undergoes phase variation under Dam methylation control. The OpvA and OpvB proteins form intertwining ribbons in the inner membrane. Synthesis of OpvA and OpvB alters lipopolysaccharide O-antigen chain length and confers resistance to bacteriophages 9NA (Siphoviridae), Det7 (Myoviridae), and P22 (Podoviridae). These phag...

A high-frequency-transducing (HFT) lysate for tetracycline resistance (Lets) was obtained by inducing an unusual tets P22 lysogen which had been made by transducing Salmonella typhimurium LT.2 to letR with P22 phage grown on an LT-2 strain carrying the R factor 222. The HFT lysate contains defective P22 particles, called P22 Tc-10, which cannot grow or lysogenize (i.e., transduce tetn) upon sin...

We have identified a novel protein, p22, required for “constitutive” exocytic membrane traffic. p22 belongs to the EF-hand superfamily of Ca-binding proteins and shows extensive similarity to the regulatory subunit of protein phosphatase 2B, calcineurin B. p22 is a cytosolic N-myristoylated protein that undergoes conformational changes upon binding of Ca. Antibodies against a p22 peptide block ...

abstract background: hepatitis b virus (hbv) infection in patients who lack detectable hepatitis b surface antigen (hbsag) is called occult hepatitis b infection. such infections have been frequently identified in patients with chronic hepatitis cliver disease, but their prevalence is not known. methods: 207 patients with chronic hepatitis c who were hcv -rna and antihcv positive were studied f...

Proteins containing the EF-hand Ca(2+)-binding motif, such as calmodulin and calcineurin B, function as regulators of various cellular processes. Here we focus on p22, an N-myristoylated, widely expressed EF-hand Ca(2+)-binding protein conserved throughout evolution, which was shown previously to be required for membrane traffic. Immunofluorescence studies show that p22 distributes along microt...

Flavocytochrome b(558), the catalytic core of the phagocytic NADPH oxidase, mediates the transfer of electrons from NADPH to molecular oxygen to generate superoxide for host defense. Flavocytochrome b is a membrane heterodimer consisting of a large subunit gp91(phox) (NOX2) and a smaller subunit, p22(phox). Although in neutrophils flavocytochrome b has been shown to localize to the plasma membr...