2015 Aim: Bioreactors are an essential component in every biotechnological process. Due to the multitude of different microbial and mammalian organisms used for production of complex products, novel concepts for customized cultivations are necessary. Results: A ceramic capillary-based bioreactor enabling a novel approach for steadystate biofilm cultivation is presented. A model for the determin...

The hybridoma 192 was used to produce a monoclonal antibody (MAb) against 17-hydroxyprogesterone (17-OHP), for possible use in screening for congenital adrenal hyperplasia (CAH). The factors influencing the MAb production were screened and optimized in a 2 L stirred bioreactor. The production was then scaled up to a 20 L bioreactor. All of the screened factors (aeration rate, stirring speed, di...

We have developed an integrated array of microbioreactors, with 100 microL working volume, comprising a peristaltic oxygenating mixer and microfluidic injectors. These integrated devices were fabricated in a single chip and can provide a high oxygen transfer rate (k(L)a approximately 0.1 s(-1)) without introducing bubbles, and closed loop control over dissolved oxygen and pH (+/-0.1). The syste...

In this study, a miniature stirred tank bioreactor was designed for treatment of waste gas containing benzene, toluene and xylene. Oxygen mass transfer characteristics for various twin and single-impeller systems were investigated for 6 configurations in a vessel with 10 cm of inner diameter and working volume of 1.77L. Three types of impellers, namely, Rushton turbine, Pitched 4blades and Pitc...

RESEARCH ARTICLE Intensifying the Fermentation of Aspergillus oryzae in a Stirred Bioreactor Using Maxblend Impeller Narges Ghobadi, Chiaki Ogino and Naoto Ohmura Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe Univer...

Virtual laboratories have successfully proven to be very versatile and intuitive when simulating experiments in science, biotechnology, engineering. These tools must complement the carried out real labs or pilot plants. This study describes creation of a virtual laboratory through Easy JavaScript Simulation platform. A web-based simulation an enzymatic stirred-tank bioreactor has been built usi...

The present work deals with the kinetics of rifamycins production and degradation by Amycolatopsis mediterranei in submerged culture using industrial medium. Cultivations were conducted in shake flasks and stirred tank bioreactor to evaluate the influence of transfer of process to higher scale on overall process kinetics and to obtain better understanding for the kinetics of rifamycins producti...

Background During the chronic myelogenous leukemia (CML) the Bcr-Abl oncoprotein is produced, which leads to unregulated cell proliferation. CML is treated with one of several targeting therapies such as imatinib, (formerly STI-571 [Glivec; Novartis, Switzerland]) a selective inhibitor that blocks tyrosin kinase activity of the Bcr-Abl oncoprotein. Apart from the second generation Bcr-Abl inhib...

Under proper operating conditions, a low dead volume continuous filtration module operated on biological broths (yeast and bacteria suspensions in stirred reactors) still fulfills the flow-rate requirements of an analytical apparatus (for example HPLC or FIA) without membrane regeneration. The filtrate stream has been successfully connected to a bioreactor in order to perform the automated HPLC...