BACKGROUND/AIMS The development of effective, accurate, and rapid diagnostic methods for Mycobacterium infection and mycobacterial species identification is required. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is an easy, rapid and inexpensive technique for identifying Mycobacterium spp. METHODS We performed PCR-RFLP to detect and identify Mycobacterium spp....

BACKGROUND & OBJECTIVES Streptococcus pneumoniae is a major cause of morbidity and mortality especially in children less than five years, particularly in India. We present data on S.pneumoniae infections in children less than five years age group, with response to its serotype distribution, antibiotic resistance profile and available vaccines expected coverage. METHODS Children aged less than...

When thinking about fluid within the body we are essentially thinking about water. Tight regulation of the balance between water intake and output, and its distribution, is vital to the optimal function of every organ system within the body. In a wide variety of illnesses and during surgery, disturbances to this fine balance occur which must be identified and corrected to prevent deterioration,...

Water is a very important component in life, such as the use of water drinking water, all living things, both humans, animals, and plants, need water. Part human body consists fluids, so helps to work carry nutrients from stomach parts body. So that hygienic source needed, through processing process first until meets health requirements. The purpose this study was detect presence Escherichia co...

BACKGROUND Duration of digestion/decontamination has a considerable impact on yield of mycobacteria especially from sterile body fluids and pus specimens. Additionally, duration of digestion/decontamination affects the contamination rates. This study evaluates the effect of digestion/decontamination protocol for 15 and 20min versus inoculation of media directly from the sample on contamination ...

Corynebacterium species have often been considered normal skin flora or contaminants; however, in recent years they have been increasingly implicated in serious infections. Moreover, many new species have been discovered and old species renamed, especially after molecular biology techniques were introduced. Corynebacterium mucifaciens is mainly isolated from blood and from other normally-steril...