Several proteomics approaches are available that are defined by the level (protein or peptide) at which analysis takes place. The most widely applied method still is bottom-up proteomics where the protein is digested into peptides that can be efficiently analyzed with a wide range of LC-MS or MALDI-TOF-MS instruments. Sample preparation for bottom-up proteomics experiments requires several trea...

The present study of a typical proteolytic enzyme extends the quantitative investigations previously reported from this laboratory on the effects of pressure on protein systems in viva (1) and in vitro (2). This paper presents new data on the self-digestion of trypsin and the digestion of casein by trypsin; a forthcoming paper will discuss the effects of temperature and hydrostatic pressure on ...

Anaerobic digestion (AD) of organic municipal solid waste (OMSW) is considered as a key element in sustainable municipal waste management due to its benefits for energy, environment, and economy. This process reduces emission of greenhouse gases, generates renewable natural gas, and produces fertilizers and soil amendments. Due to its advantages over other treatment methods and waste-to-energy ...

A cell-free extract of Saccharomyces cerevisiae containing the angiotensin I-converting enzyme (ACE) inhibitory peptide was treated in a successive simulated gastric-intestinal bioreactor (step 1: amylase digestion, step 2: gastric fluid digestion, step 3: intestinal fluid digestion) to illustrate the absorption pattern of antihypertensive ACE inhibitory peptide, and the ACE inhibitory activiti...

Whether anaesthetic technique during potentially curative cancer surgery influences recurrence is a question that dates to two publications about 15 yr ago, each of which presented associations between use regional anaesthesia and improved outcomes.1Exadaktylos A.K. Buggy D.J. Moriarty D.C. Mascha E. Sessler D.I. Can anesthetic for primary breast affect or metastasis?.Anesthesiology. 2006; 105:...

A universal, rapid DNA assembly method for efficient multigene plasmid construction is important for biological research and for optimizing gene expression in industrial microbes. Three different approaches to achieve this goal were evaluated. These included creating long complementary extensions using a uracil-DNA glycosylase technique, overlap extension polymerase chain reaction, and a SfiI-b...