نتایج جستجو برای: specific primer
تعداد نتایج: 1061857 فیلتر نتایج به سال:
The analytical performance of 9 different PCR primer sets designed to detect Escherichia coli and Shigella in water has been evaluated in terms of ubiquity, specificity, and analytical detection limit. Of the 9 PCR primer sets tested, only 3 of the 5 primer sets targeting uidA gene and the primer set targeting tuf gene amplified DNA from all E. coli strains tested. However, of those 4 primer se...
To provide feasible primer sets for performing a polymerase chain reaction (PCR) experiment, many primer design methods have been proposed. However, the majority of these methods require a relatively long time to obtain an optimal solution since large quantities of template DNA need to be analyzed. Furthermore, the designed primer sets usually do not provide a specific PCR product size. In rece...
Background: Celiac Disease (CD) is a T cell-mediated disorder. Recent studies suggest the role of chemokines CXCL10 and CXCL11 to promote the arrival of cells into inflamed tissues and in lymphocytic recruitment in active CD. The aim of this study was to investigate the new specific primer pairs for analysis of human CXCL10 and CXCL11 genes in blood samples of CD patients by Polymerase Chain Re...
the sigatoka disease complex of banana involves three related ascomycetous fungi viz., mycosphaerella fijiensis, m. musicola, and m. eumusae. due to rather similar symptoms on banana, similar teleomorph morphology and problems associated with growth and sporulation in culture, species identification based on classical methods is troublesome. the exact distribution of these three species and the...
The CYP2C subfamily of the cytochrome P450 gene superfamily encodes heme-thiolate proteins that have a myriad of biologic functions. CYP2C proteins detoxify xenobiotics and metabolize endogenous lipids such as arachidonic acid to bioactive eicosanoids. We report new methods and results for the quantitative polymerase reaction (qPCR) analysis for the 15 members of the mouse Cyp2c subfamily (Cyp2...
Real-time quantitative polymerase chain reaction (qPCR) is one of the most important methods for analyzing the expression patterns of target genes. However, successful qPCR experiments rely heavily on the use of high-quality primers. Various qPCR primer databases have been developed to address this issue, but these databases target only a few important organisms. Here, we developed the qPrimerD...
This service integrates primer designing code from the popular Primer3 1 software with a specificity check that uses a custom BLAST search. Primer-BLAST eliminates the need to design primers at another site and then perform a search with those primers using the main NCBI BLAST service to check specificity. Moreover, Primer-BLAST can design primers that amplify only a particular splice variant o...
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