Background: Several studies have examined the presence of DNA methylation of CpG islands in leukemia. Methylation of SOX17 and RUNX3 genes may play a role in leukemogenesis through silencing tumor suppressor genes. We investigated the methylation status of SOX17 and RUNX3 genes in patients with acute leukemia.    Methods: In this case-control study, peripheral blood samples from 100 AML and 10...

Fetal stem cells differ phenotypically and functionally from adult stem cells in diverse tissues. However, little is known about how these differences are regulated. To address this we compared the gene expression profiles of fetal versus adult hematopoietic stem cells (HSCs) and discovered that the Sox17 transcriptional regulator is specifically expressed in fetal and neonatal but not adult HS...

Embryonic stem (ES) cells offer a valuable source for generating insulin-producing cells. However, current differentiation protocols often result in heterogeneous cell populations of various developmental stages. Here we show the activin A-induced differentiation of mouse ES cells carrying a homologous dsRed-IRES-puromycin knock-in within the Sox17 locus into the endoderm lineage. Sox17-express...

Sox7, Sox17 and Sox18 constitute group F of the Sox family of HMG box transcription factor genes. Dominant-negative mutations in Sox18 underlie the cardiovascular defects observed in ragged mutant mice. By contrast, Sox18(-/-) mice are viable and fertile, and display no appreciable anomaly in their vasculature, suggesting functional compensation by the two other SoxF genes. Here, we provide dir...

RATIONALE Vascular endothelial growth factor (VEGF) signaling is a key pathway for angiogenesis and requires highly coordinated regulation. Although the Notch pathway-mediated suppression of excessive VEGF activity via negative feedback is well known, the positive feedback control for augmenting VEGF signaling remains poorly understood. Transcription factor Sox17 is indispensable for angiogenes...

the foregut endoderm and the defective expansion of the midand hindgut at early somite stages. However, there remains unclear whether or not Sox17 is required for the subsequent development, maturation and maintenance of the endoderm derivatives such as livers and pancreas due to the early embryonic lethal of Sox17-null mutant embryos before 10.0 dpc. Recently, we noticed the neonatal lethality...

Congenital anomalies of the kidney and the urinary tract (CAKUT) represent a major source of morbidity and mortality in children. Several factors (PAX, SOX,WNT, RET, GDFN, and others) play critical roles during the differentiation process that leads to the formation of nephron epithelia. We have identified mutations in SOX17, an HMG-box transcription factor and Wnt signaling antagonist, in eigh...

the foregut endoderm and the defective expansion of the midand hindgut at early somite stages. However, there remains unclear whether or not Sox17 is required for the subsequent development, maturation and maintenance of the endoderm derivatives such as livers and pancreas due to the early embryonic lethal of Sox17-null mutant embryos before 10.0 dpc. Recently, we noticed the neonatal lethality...

INTRODUCTION SOX17 promoter methylation can provide important prognostic information in cancer. We developed a novel semi-quantitative MS-HRMA assay for SOX17 promoter methylation. METHODS The assay was optimized by using synthetic control samples and validated by analyzing 165 clinical samples: a) 107 formalin fixed paraffin embedded (FFPEs) samples of patients with early breast cancer, b) 2...

the foregut endoderm and the defective expansion of the midand hindgut at early somite stages. However, there remains unclear whether or not Sox17 is required for the subsequent development, maturation and maintenance of the endoderm derivatives such as livers and pancreas due to the early embryonic lethal of Sox17-null mutant embryos before 10.0 dpc. Recently, we noticed the neonatal lethality...