The red cells of patients with chronic hemolytic anemia due to cold agglutinins are agglutinated by antiglobulin serum in a nongamma reaction due to the coating of beta-globulins, C'4 and C'3. The red cells of such patients are abnormally resistant to C' hemolysis by cold agglutinin. Normal red cells can be made equally resistant to C' hemolysis by incubation with cold agglutinin and normal ser...

1. Protective substances and agglutinins were not demonstrated in the blood of patients with pneumonia untreated with serum before the fall in the temperature by crisis or lysis. 2. Protective substances and agglutinins were frequently demonstrated in serum treated patients during the course of the disease. 3. Protective substances and agglutinins were usually present at the time of the tempera...

1. There is no demonstrable amount of agglutinin for Treponema pallidum (Noguchi) in normal human and normal rabbit serum in dilutions as low as 1:20. 2. Agglutinins for Treponema pallidum are readily produced in young rabbits by the administration of pure cultures of living spirochetes. 3. There is no appreciable amount of agglutinin for Treponema pallidum culture used in the sera of secondary...

We studied the value of the Widal tube agglutination test for the diagnosis of typhoid fever. The subjects were all adults >18 years of age and were divided into four groups: (i) 317 healthy blood donor controls, (ii) 31 bacteriologically confirmed patients with Salmonella enterica serotype Typhi, (iii) 21 patients with a clinical diagnosis of typhoid fever, and (iv) 41 febrile nontyphoid patie...

Chicken serum promotes migration of cardiac mesenchymal cells of chick embryos in vitro. In the present study, migration promotion of unknown migration promoters in chicken serum was examined by using lectins. Cardiac mesenchymal cells of the conotruncal and atrioventricular cushions were cultured on collagen type-I gel with medium including chicken serum. A concentration (100 micrograms/ml) of...

Cold Agglutinin Disease is a hemolytic anemia associated with cold reactive autoantibodies. Although the acute form of cold agglutinin disease can be attributed to autoimmune or infectious diseases and lymphoproliferative diseases, it has,to the best of our knowledge, so far,never been reported as secondary to Mycoplasma pneumonia in a type 2 diabetic individual. In this paper, we report a case...

Lymphocytes that formed rosettes with sera made to the serum macroglobulins human red cells in the cold were observed detected the same proteins on the lymphoin the peripheral blood of four patients cyte surface. Evidence was obtained that with chronic cold agglutinin disease. Simthe rosette test underestimated the numilar rosettes were not observed in normals ber of cells bearing the specific ...

Porous membranes with glycopolymer brushes were prepared as biomaterials for affinity separation. Glycopolymer brushes contained acrylic acid and D-mannose or N-acetyl-D-glucosamine, and were formed on substrates by surface-initiated atom transfer radical polymerization. The presence of glycopolymer brush was confirmed by X-ray photoelectron spectroscopy, contact angle, and ellipsometry measure...

The development of a solid-phase radioimmunoassay procedure for the detection of Candida albicans antigens in serum of mice is described. Antibodies against C. albicans that were used in the radioimmunoassay procedure were partially purified from immune serum by a C. albicans antigen-coupled affinity column. Elution of anti-C. albicans antibodies from the column was by glucose and mannose; 4 mg...