in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...

We present a profile-profile multiple alignment strategy that uses database searching to collect homologues for each sequence in a given set, in order to enrich their available evolutionary information for the alignment. For each of the alignment sequences, the putative homologous sequences that score above a pre-defined threshold are incorporated into a position-specific pre-alignment profile....

case report: recently a 20-day-old layer flock with mortality has been submitted to the pcr lab. infectious bronchitis virus (ibv) has been detected in the clinical samples. results: a phylogenetic tree based on a partial s1 gene sequence showed iranian ibv variant located in lx4-type cluster. this cluster include all qxibv-type detected in china and european countries. the highest sequence hom...

inhalation of pollens from different species of acacia is a common cause of respiratory allergy in tropical areas of the world. acacia farnesiana is commonly used as street trees in towns and ornamental shade trees in parks and gardens throughout arid and semi-arid regions of asia. this study aimed to produce and purify the a. farnesiana pollen profilin (aca f 2) and evaluate its nucleotide seq...

We use the Dolbeault cohomology to investigate Koszul–Brylinski homology on holomorphic Poisson manifolds. obtain Leray–Hirsch theorem for Hochschild and Mayer–Vietoris sequence, Künneth homology. In particular, we show some relations of homologies around a blow-up transformation general case (not necessarily compact) by our previous works cohomology.

The Euler characteristic is an invariant of a topological space that in precise sense captures its canonical notion size, akin to the cardinality set. closely related homology space, as it can be expressed alternating sum Betti numbers, whenever well-defined. Thus, one says categorifies characteristic. In his work on generalisation cardinality-like invariants, Leinster introduced magnitude metr...