Source and Description of Probe. A 2.7-kb human cDNA clone for TAP2 was excised from the XbaI site of the plasmid pRSV.5neo (Bahram et al., 1991). Method of Detection. DNA was isolated from whole blood and digested with TuqI. Fragments were separated by agarose gel electrophoresis and transferred to charged nylon membranes. Hybridizations were for 16 to 20 h at 65°C (10% dextran sulfate, 7% SDS...

Chelating agents’ application for well stimulation is gaining more and interest, as they can perform under harsh conditions. However, the mutual influence of surfactants chelating agents on wettability alteration hydrophobic carbonate rock conditions high-temperature relatively unexplored. This paper aims to study interfacial processes surface in presence EDTA-based agent different classes. Cat...

Biological biomaterials for tissue engineering purposes can be produced through tissue and/or organ decellularization. The remaining extracellular matrix (ECM) must be acellular and preserve its proteins and physical features. Placentas are organs of great interest because they are discarded after birth and present large amounts of ECM. Protocols for decellularization are tissue-specific and ha...

The techniques available for the identification of plasmid DNA in gram-positive bacteria such as mycelium-forming Streptomycetes are time consuming. Nearly all methods described require the cultivation of the bacteria in liquid medium (1). Simple plasmid isolation techniques have been developed for gramnegative bacteria (2) but they are not applicable to gram-positive prokaryotes. Here, we pres...

The effect of EDTA on Salmonella enterica serovar Typhimurium was studied in different growth phases with cells grown with or without Ca(2+) and Mg(2+) supplementation. EDTA affected the outer membrane much more strongly in the early exponential phase than in the mid- or late exponential phase, as indicated by uptake of 1-N-phenylnaphthylamine (a nonpolar hydrophobic probe, M(r) 219), and deter...

The objective of this research was to evaluate the effect sperm on chromatin stability and its relationship with membrane integrity structural – physiological rate fertilization female sheep. Ejaculates (2 × 109 sperm·mL-1) 70% motility were collected using an artificial vagina (n=5, 2 years old. For this, each ram served fifteen sheep (n=75), generating thus five different Groups (A, B, C, D, ...

We evaluated the inducibility of nitrate-dependent Fe(II)-EDTA oxidation (NDFO) in non-growth, chloramphenicol-amended, resting-cell suspensions of Dechloromonas sp. strain UWNR4 and Acidovorax sp. strain 2AN. Cells previously incubated with Fe(II)-EDTA oxidized ca. 6-fold more Fe(II)-EDTA than cells previously incubated with Fe(III)-EDTA. This is the first report of induction of NDFO by Fe(II).

background: the aim of present study is to evaluate the effect of polyvinyl pyrrolidone (pvp) routinly used during icsi procedure on sperm membrane integrity, and sperm chromatin status. materials and methods: this study was carried out on 21 semen samples from the infertile men referring to isfahan fertility and infertility center. the processed semen samples were divided into two portions. on...

labeled membrane proteins by density gradient ultracentrifugation using 1 O%-40% sucrose gradients containing the nonionic detergent. Studies were performed using soluble proteins derived from membranes isolated in the presence or absence of EDTA. Analysis of gradient fractions by SDS-polyacrylamide gel electrophoresis showed that in the absence of divalent cation chelation. GP lIb and lIla pen...

labeled membrane proteins by density gradient ultracentrifugation using 1 O%-40% sucrose gradients containing the nonionic detergent. Studies were performed using soluble proteins derived from membranes isolated in the presence or absence of EDTA. Analysis of gradient fractions by SDS-polyacrylamide gel electrophoresis showed that in the absence of divalent cation chelation. GP lIb and lIla pen...