The rumen microbial ecosystem is a complex system where rumen fermentation processes involve interactions among microorganisms. There are important relationships between diet and the ruminal bacterial composition. Thus, we investigated the ruminal fermentation characteristics and compared ruminal bacterial communities using tag amplicon pyrosequencing analysis in Yanbian yellow steers, which we...

Megasphaera elsdenii belongs to the group comprising the ruminal and intestinal lactate- and sugar-fermenting species. In the present study the fermentation characteristics, metabolism of glucose and lactate, and susceptibility to antimicrobial agents of four ruminal strains were investigated. Particular attention was given to the mixed-substrate fermentation pattern and resultant fermentation ...

Greenhouse gases have been of serious global concern to environmentalists. Enteric ruminal fermentation and manure are seen to be responsible for global warming. Based on a better understanding of positive tan-nin effects on ruminant nutrition, the feeding value of browse trees and shrubs containing tannin, their roles on methanogenic rumen microbes in overcoming the production of enteric ferme...

Hofírek B. , D. Haas: Comparative Studies of Ruminal Fluid Collected by Oral Tube or by Puncture of the Caudoventral Ruminal Sac. Acta Vet. Brno 2001, 70: 27-33. Samples of ruminal fluid were collected in four groups of high-producing dairy cows simultaneously by an oral probe and by percutaneous puncture of the caudoventral ruminal sac. The samples were tested for basic indicators of the metab...

Assessments of an Exogenous Proteolytic Enzyme in Beef Steer Diets to Improve Growth Performance and Ruminal fermentation

Much interest has been generated over the past few years aimed at evaluating alternative means to manipulate the gastrointestinal microflora in production livestock. Motivation for examining these alternatives comes from increasing public scrutiny about the use of antibiotics in the animal feed industry. However, compared to the amount of information available detailing the effects of antimicro...

This study investigates the effects of three branched-chain amino acids (BCAA; valine, leucine, and isoleucine) on the in vitro ruminal fermentation of wheat straw using batch cultures of mixed ruminal microorganisms. BCAA were added to the buffered ruminal fluid at a concentration of 0, 2, 4, 7, or 10 mmol/L. After 72 h of anaerobic incubation, pH, volatile fatty acids (VFA), and ammonia nitro...

Four ruminally cannulated Holstein cows were used in a 4 × 4 Latin square design to determine the effect of feeding linted, delinted and extruded linted whole cottonseed (WCS) on nutrient digestibility and ruminal fermentation. Result showed that crude protein digestibility was lower in linted and delinted WCS treatments than control and extruded linted WCS treatment. Cows fed extruded delinted...

To investigate the effects of synchronizing nonstructural carbohydrate (NSC) and protein degradation on intake and rumen microbial fermentation, four ruminally fistulated Holstein heifers (BW = 132.3 +/- 1.61 kg) fed high-concentrate diets were assigned to a 4 x 4 Latin square design with a 2 x 2 factorial arrangement of treatments studied in vivo and in vitro with a dual-flow continuous cultur...

This study aimed to investigate the effects of ruminal infusion of garlic oil (GO) on fermentation dynamics, fatty acid (FA) profile, and abundance of bacteria involved in biohydrogenation in the rumen. Six wethers fitted with ruminal fistula were assigned to two groups for cross-over design with a 14-d interval. Each 30-d experimental period consisted of a 27-d adaptation and a 3-d sample coll...