HS (heparan sulphate) has hitherto not been found on human red blood cells (RBCs, erythrocytes). However, malarial-parasite (Plasmodium falciparum)-infected RBCs adhere to uninfected RBCs via HS-like receptors. In the present paper we demonstrate that human RBCs carry epitopes for an anti-HS antibody. Glycans isolated from RBC membranes reacted to HS-specific degradations and adhered to an HS-b...

The effect of nanoparticles on the mechanical behavior red blood cells (RBCs) under an extensional flow has not been extensively studied. In this work, by using a microfluidic hyperbolic contraction, it is assessed deformability healthy RBCs in contact with ∼20 nm magnetic iron and non-magnetic cerium. results showed that, controlled flow, index depended cells’ velocity along contraction. Addit...

Because of the imbalance in the supply and demand of red blood cells (RBCs), especially for alloimmunized patients or patients with rare blood phenotypes, extensive research has been done to generate therapeutic quantities of mature RBCs from hematopoietic stem cells of various sources, such as bone marrow, peripheral blood, and cord blood. Since human embryonic stem cells (hESCs) and induced p...

Athletes may undergo blood transfusion to increase their red cell mass and the oxygen carrying capacity of their blood in order to confer a competitive advantage. Allogeneic transfusions are normally mismatched at one or more minor blood group antigens. The most sensitive and accurate method known to detect this form of blood doping is flow cytometry. Low percentages of antigen-positive and ant...

Beyond their established role as oxygen carriers, red blood cells have recently been found to contribute systemic NO and sulfide metabolism act potent circulating antioxidant cells. Emerging evidence indicates that reactive species derived from the of O2, NO, H2S can interact with each other, potentially influencing common biological targets. These interactions encompassed in concept interactom...

Introduction: The Dutch naturalist Jan Swammerdam was the first individual to microscopically observe red blood cells (RBCs) or erythrocytes in the year 1658. Credit for the discovery of RBCs, however, often goes to Anton van Leeuwenhoek, another Dutch scientist who made the first recorded illustration of RBCs in 1695, writing, “I have observed, taking some blood out of my hand, that it consist...

Background: Glycophorin A (GPA) is one of two glycoproteins found on the surface human red blood cells (RBCs) that constitute MNS group system. The structure GPA’s extracellular domain unknown despite previous attempts using X-ray crystallography and NMR spectroscopy. As a result, there knowledge gap regarding antigen presentation. This hinders production monoclonal antibodies (mAbs) against an...

We have tested a modification of the Brookhaven method for in vitro labeling of red blood cells (RBCs) with technetium-99m by adding an initial centrifugation step and performing the labeling on packed RBCs. This results in reproducible, high labeling efficiencies (99.3% ± 0.4%, n =50) after 15 min of incubation. The use of packed RBCs also results in a higher concentration of labeled RBCs (sma...

Red blood cells (RBCs) are known to form aggregates in the form of rouleaux due to the presence of plasma proteins under physiological conditions. The formation of rouleaux can also be induced in vitro by the addition of macromolecules to the RBC suspension. Current data on the adhesion strength between red blood cells in their natural discocyte shapes mostly originate from indirect measurement...